Protein Info for mRNA_5758 in Rhodosporidium toruloides IFO0880

Name: 14126
Annotation: K00627 DLAT, aceF, pdhC pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 1 to 64 (64 residues), 59.9 bits, E=2.7e-20
• TIGR01349: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase
  • amino acids 1 to 443 (443 residues), 521.9 bits, E=8.3e-161
• PF02817: E3_binding
  • amino acids 137 to 172 (36 residues), 57.3 bits, 2.3e-19
• PF00198: 2-oxoacid_dh
  • amino acids 201 to 442 (242 residues), 258 bits, E=1.2e-80

Best Hits

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 60% identity to uma:UM00594.1)

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (2/3 steps found)
• pyruvate fermentation to acetate V (2/3 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (2/4 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (16/26 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (4/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (8/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.12

Use Curated BLAST to search for 2.3.1.12

Protein Sequence (443 amino acids)

>mRNA_5758 K00627 DLAT, aceF, pdhC pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) (Rhodosporidium toruloides IFO0880)
MTEGGIAQWKKKEGESFAPGDVLLEIETDKATMDVEAQDEGVLGKILVGDGSKAVQVGSP
VAVIGEEGDDFSEEQIQFVLKEAPSGGDVAEPKQESKQEKKPEEPKKEEPKKEEAAPKKQ
ESKKQSSLELSSDRPIILASPMAKRLALENGVPLAKVKGTGPDGRILAEDVEKYKASAPS
AAAAGPAAAAKASPAAGAGAAYTDTPVSNMRRTIASRLTESKSGTPHYYLTAEINMDRVN
KLREAFNTAAKAADAAGASKDGVKGGVKLSVNDFIVKASALALQDVPEVNSGWHGDFIRE
YATQDICVAVATPNGLITPIVADAGRKGLATISSQAKQLAAKARDGKLKPEEYQGGSFTI
SNLGMMGIESFTAIINPPQSCILAIGASEKKLVLDPASEKGFKEVSVMKATLSCDHRVVD
GAVGARWMKAFKSYLESPLSFML