Protein Info for mRNA_5890 in Rhodosporidium toruloides IFO0880

Name: 14258
Annotation: K01876 DARS, aspS aspartyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 TIGR00458: aspartate--tRNA(Asn) ligase" amino acids 176 to 636 (461 residues), 522.4 bits, see alignment E=4.8e-161 PF00152: tRNA-synt_2" amino acids 305 to 630 (326 residues), 267 bits, see alignment E=1.1e-83

Best Hits

Swiss-Prot: 58% identical to SYDC_YEAST: Aspartate--tRNA ligase, cytoplasmic (DPS1) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 61% identity to cne:CND06300)

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (636 amino acids)

>mRNA_5890 K01876 DARS, aspS aspartyl-tRNA synthetase (Rhodosporidium toruloides IFO0880)
MLATCLTRTGHRASLAPLARSLTLQRSHSHLASSTHTTTMTGSGLHSILDKVIPHHHSRS
NSTESSRATSPTPTGTGSKTPLSAATSRKSVDSKHSSSHEIVLGENGEPLSKSELKKRQK
AEEKERLKQEKAKKIAEKEAAEKAAKQQAEEDYSPNSYGKLPLNNSSERTGAKREKFEDI
SAAREGETVLVRARLQTSRAQGAKMVFVVLRQQAHTLQGLVVVEPEHVSKKMVRWVESIH
DESIVLVEGVVQKAKEPIKSCTIHDVELKIRKIFVVSEAPAMLPFTINDALRPMDAEDGV
QVALDTRLDNRVLDLRTITNQAIFRIQSGVGNLFREYLNTQGFTEIHTPKLQGAATESGA
SVFRVSYFKTIAFLAQSPQLHKQMCIAADMEKVYEIGPVFRAEDSNTHRHLTEFMGLDLE
MAFEEHYHEAMETIDGMLKHVFAGLKQRYAEEIGVVRKQFPHDEFTWLPDTLVLKHTEAI
KLLQDAGVQQGTPPQPIGDYDDMNTASEKALGKIVKEKYGTDYYIIDKFPLEIRPFYTMP
DPSNPKLSNSYDFFMRGEEILSGAQRVHDAALLEERIKSVGINPDDMKPYVDAFRLGCPP
HAGGGIGLERVVMLFLQINNIRRASLFPRDPKRTSP