Protein Info for mRNA_6546 in Rhodosporidium toruloides IFO0880

Name: 14914
Annotation: K01702 LEU1 3-isopropylmalate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 751 TIGR00170: 3-isopropylmalate dehydratase, large subunit" amino acids 4 to 472 (469 residues), 804.4 bits, see alignment E=2.7e-246 PF00330: Aconitase" amino acids 9 to 465 (457 residues), 545.4 bits, see alignment E=1.4e-167 PF00694: Aconitase_C" amino acids 530 to 653 (124 residues), 125.3 bits, see alignment E=2.1e-40 TIGR00171: 3-isopropylmalate dehydratase, small subunit" amino acids 530 to 715 (186 residues), 231.4 bits, see alignment E=7.6e-73

Best Hits

KEGG orthology group: K01702, 3-isopropylmalate dehydratase [EC: 4.2.1.33] (inferred from 63% identity to cci:CC1G_00469)

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33

Use Curated BLAST to search for 4.2.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (751 amino acids)

>mRNA_6546 K01702 LEU1 3-isopropylmalate dehydratase (Rhodosporidium toruloides IFO0880)
MSQPKTLYDKVYDNHVVREDENGSTILYIDRHIVHEVTSPQAFEGLSKAGRKVRRLDGTL
ATVDHNVPTSSRKNYTDSASFIKEADSRIQVLTLEENVKKFGVQFFGMTDRRQGIVHVIG
PEQGFTLPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTILQSKSKNMRVQIDG
ELAPGVNSKDVMLHVIGTIGTAGGNGSVIEFCGTTVEAMSMEARMSMANMSIEAGARAGM
FAPDEITFDYLKGKPLAPQGAEWDKAVEFWKSLKSDEGAHYDKVVKINAADIPPTVTWGT
SPEDTVSITGTVPDPAQAPNDAAKAKIERALTYMGLEPNQKIQDIKVTNVFIGSCTNSRI
EDLRSAASIVKGRKVAEGVYAMVVPGSGLVKHQAEQEGLDRVFSEAGFDWREAGCSMCLG
MNPDMLSPGQRCASTSNRNFEGRQGPGGRTHLMSPAMAAAAAIAGHLADARKYQPDERTG
AAPVPAVEIAPEEQQVPTTQQTPDAADDGVAQSLGAPEVTAPVTTAAAGMPKFEVVEGAA
APLERANVDTDLIIPARFLKTIKRTGLGVSLFAALRYNADGSENPDFILNKPGYRDSKIL
VVSGGNFGCGSSREHAPWALLDFGIRAVIASSFADIFNNNMYKNGSLPVILPEEQIAILI
EDAKAGKPLAVDLPKQVVRRQNGDEYPFEVDQFRKHCLVNGLDEISLTLQHEDSISQYEK
KRSELWPWLDGKTYRAGKIGVAGGAKTLTDW