Protein Info for mRNA_6664 in Rhodosporidium toruloides IFO0880

Name: 15032
Annotation: K03165 TOP3 DNA topoisomerase III

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1007 PF01751: Toprim" amino acids 6 to 155 (150 residues), 61 bits, see alignment E=2.4e-20 PF01131: Topoisom_bac" amino acids 174 to 585 (412 residues), 347.1 bits, see alignment E=3.2e-107 PF06839: zf-GRF" amino acids 724 to 766 (43 residues), 54 bits, see alignment (E = 2.8e-18) amino acids 804 to 846 (43 residues), 45 bits, see alignment (E = 1.9e-15) PF00098: zf-CCHC" amino acids 877 to 894 (18 residues), 29 bits, see alignment (E = 1.7e-10) amino acids 916 to 932 (17 residues), 18.6 bits, see alignment (E = 3.2e-07) amino acids 952 to 967 (16 residues), 22.3 bits, see alignment (E = 2.1e-08)

Best Hits

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1007 amino acids)

>mRNA_6664 K03165 TOP3 DNA topoisomerase III (Rhodosporidium toruloides IFO0880)
MPAKWLCVAEKPSVAKSIAQILSGGQFTTRTARKPQWIKNYSFSYRQGQQGGHSDFTVTS
VAGHLTSSDFDDQYRKWHSCDPRDLFDAGIRTFVTQDADHKGIEANLLAEARNATHLMIW
TDCDREGEYIGSVVAEVCRKAKPNIVVKRARFSAIIPAQINQAARDPVDLDLLQAAAVGA
RMELDLRVGSIFTRVQTLELQRQVGALEDSLISYGPCQFPTLGFVVDQYERVSSFVPEPF
WYIHVALAREDSKTTFSWRRGRLYDRNVAQALLDIVEDAGEARVTKWETKPTQKWKPLPL
TTVELQKSGSRLLRMPPKRVLEIAESLYHKGILSYPRTETDQFDRDFNFHELIAQHKGDN
AWGTFATNLLDNGGFERPRNGKKNDKAHPPIHPTQHVNNLTGDDKRVYDLVVRRFLGCCS
KNAKGLETTCEIEIASEAFSAKGLVIRERNYLDVYIYDKWTGNHLPEFQVRERFEPDELS
LKEGMTTQPSLLTEADLVSLMDKNGIGTDATIAEHISKIIDREYVIKQKEGSVEYLVPST
LGIGLVKAYDALGLENSLSKPHLRRLTEERLVQICEGKKNKADVITQSLNEYREVLFRTQ
QQMAAFVATVRQYVEGNLGQGQGGGWGGNDSDGDDFGGGGGGPRKRTTQKKPAAKRTAAK
TAAGTRGKAASAGKTGAKSTAAKRKKKEDEDEGESPFSPPLTLVLTLSSADFAFDDAADQ
DVPRCGCSIPAVERTVTKDGPNKGRKFFGCSKGMSDGCGFFAWADEEGSGGSSGRKSGGG
AVVPQKRPLRQPTRKSDASDELRCDCDLTPQRLTVKAEGPNQGREFLKCPNVSKNAQCKF
FAWVDSLDGDAGPAPARGRSNSMQGGGGGGGGGGASGTCFQCGEAGHWASDCPQAGKAAK
MTRKSGGSASGGASGECYICHEEGHWASACPQRGQGGGVGASGGGGGSNYSCYKCGEQGH
FSNACPNADSTGASASRGRGRGRGSRGAGKARGRGRGGKRTVSNDWD