Protein Info for mRNA_7115 in Rhodosporidium toruloides IFO0880

Name: 15483
Annotation: K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 633 PF02219: MTHFR" amino acids 23 to 301 (279 residues), 355 bits, see alignment E=1.6e-110 TIGR00677: methylenetetrahydrofolate reductase" amino acids 29 to 303 (275 residues), 365.8 bits, see alignment E=7.3e-114

Best Hits

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.20

Use Curated BLAST to search for 1.5.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (633 amino acids)

>mRNA_7115 K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH) (Rhodosporidium toruloides IFO0880)
MTTTPELRSSHPSTKVVDRIAQLDPSKPFYTFEIFPPKTDAGLVNLLDRIDRMSALNPAW
MHVTWGAGGSTQERSLQLAGESQSMGLETCLHLTCTNLEEGVLDRTLERAKELGVVNLLA
LRGDPPRGEEYWTASSDKFQHATDLIKYIRKEYGDDFCIGVAGYPEGHADSADKLADVEY
LFEKQQAGADFVVTQLFYDVGVFMEWYRACRKRGITIPIIPGIMPIQNYLSFRRMTNLCG
THIPPQILDDLQRIQHDDAAVKEYGVQLAVKMMRELFENDIRGFHLCTLNLEKSVTRVLE
LLQWVEPGSTKISARLRKGLSPNGAPLPKVINSLRPAPSTGANVAGPDIARKDSPASWDE
FPNGRFGDARSPAYGEMDGYGVSLKLPPGEAMRQWGQPTALSDVSRVFSSYLRGSLPSNP
WSEEPLRAETEMISSHLIEMNDKRHWWTVGSQPAVDGAPSADSVHGFGPKGGYVYQKAFV
EFFLTKEELDELERKSDEDERRRRESGEGDDGLIKWFAGNAKGDFRCNMGKGDVNAVTWG
VFGAKEIVTTTLIEEMSFRAWCEEAFALWNEWSLLYPANSPSRTFIRSLADSRWLVSVVH
HDYKEKDALWKWLLALKRPEEAQGAANGEAEGR