Protein Info for mRNA_7174 in Rhodosporidium toruloides IFO0880

Name: 15542
Annotation: K11131 DKC1, NOLA4, CBF5 H/ACA ribonucleoprotein complex subunit 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 490 PF08068: DKCLD" amino acids 23 to 80 (58 residues), 107 bits, see alignment 7.9e-35 TIGR00425: putative rRNA pseudouridine synthase" amino acids 30 to 355 (326 residues), 550.4 bits, see alignment E=1.3e-169 PF01509: TruB_N" amino acids 84 to 200 (117 residues), 72.9 bits, see alignment E=7.6e-24 PF16198: TruB_C_2" amino acids 201 to 267 (67 residues), 81 bits, see alignment E=1.1e-26 TIGR00451: uncharacterized domain 2" amino acids 261 to 345 (85 residues), 39.1 bits, see alignment E=7.2e-14 PF01472: PUA" amino acids 271 to 343 (73 residues), 76.7 bits, see alignment E=2.2e-25

Best Hits

Swiss-Prot: 70% identical to CBF5_CANAL: H/ACA ribonucleoprotein complex subunit CBF5 (CBF5) from Candida albicans (strain SC5314 / ATCC MYA-2876)

KEGG orthology group: K11131, H/ACA ribonucleoprotein complex subunit 4 [EC: 5.4.99.-] (inferred from 70% identity to cim:CIMG_06375)

Predicted SEED Role

"tRNA pseudouridine 55 synthase (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.-

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (490 amino acids)

>mRNA_7174 K11131 DKC1, NOLA4, CBF5 H/ACA ribonucleoprotein complex subunit 4 (Rhodosporidium toruloides IFO0880)
MSVAQTDGDFAIKPESATPTLNTKDWPLLLKNYDKLLVRSAHFTPIPAGSSPLKRDLKSY
IKLGVINLDKPSNPSSHEVVAWIRRILRCEKTGHSGTLDPKVTGCLTVCIDRATRLVKSQ
QGAGKEYVCVLRLHGGLQSEKQLARAIETLTGALFQRPPLISAVKRQLRIRTIYDCKLIE
FDNDRHLAVFWVSCEAGTYIRTLCVHLGLLLGVGGHMQELRRVRSGAMSEADDIVTMHDV
LDAQWLYDNTRDESYLRRVIRPLESLLTGYKRIVVKDSAVNAICYGAKLMIPGLLRYEAD
IELNEEVVLMTTKGEAIAIGIAQMTTVELSTCDHGVVAKVKRCIMDRDTYPRKWGLGPTA
QNKKKMVKDGKLDKHGRTNDQTPSEWKTTYVDYSVQGEGLPAQQIPTQPTPASAAPKESE
AVRPEAVPVPAGSTEEKAVEDVAASPSKKERKDKKEKKREREGETEEERAARKKAKKAEK
ERRKSAAADA