Protein Info for mRNA_7345 in Rhodosporidium toruloides IFO0880

Name: 15713
Annotation: K00264 GLT1 glutamate synthase (NADPH/NADH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00310: GATase_2
  • amino acids 79 to 516 (438 residues), 575.9 bits, E=2.3e-176
• PF04898: Glu_syn_central
  • amino acids 553 to 837 (285 residues), 361.7 bits, E=1.5e-111
• PF01645: Glu_synthase
  • amino acids 906 to 1269 (364 residues), 512.8 bits, E=3.1e-157
• PF01493: GXGXG
  • amino acids 1350 to 1542 (193 residues), 284 bits, E=2.7e-88
• TIGR01317: glutamate synthase, NADH/NADPH, small subunit
  • amino acids 1654 to 2138 (485 residues), 775.7 bits, E=9.9e-238
• PF14691: Fer4_20
  • amino acids 1674 to 1784 (111 residues), 72.8 bits, 9.7e-24
• PF07992: Pyr_redox_2
  • amino acids 1797 to 2110 (314 residues), 77.5 bits, E=5.4e-25
• PF13450: NAD_binding_8
  • amino acids 1801 to 1834 (34 residues), 30.9 bits, see alignment (E = 1.3e-10)
• PF01593: Amino_oxidase
  • amino acids 1807 to 1834 (28 residues), 24.4 bits, see alignment (E = 9.1e-09)

Predicted SEED Role

"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• superpathway of L-citrulline metabolism (10/12 steps found)
• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamate biosynthesis III (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (3/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.13

Protein Sequence (2169 amino acids)

>mRNA_7345 K00264 GLT1 glutamate synthase (NADPH/NADH) (Rhodosporidium toruloides IFO0880)
MAPTLLHNALLVNHQKKHVSFDDDALARLSGSESSGSGATLDDALAEEEDHAVDVVRDAY
GSLPAKQGLYNPEEEKDSCGVGFVCHIKGKPSHKIVSDAKHLLCNMTHRGATGADSRDGD
GAGVMVGLPHAFMVREGEREIGHKLPGQGEYAVGNVFFRKPSSPDWHNDQTLQEQQKTFA
DIADSLDLRVLGWRHVPTDGTILGPAALSREPLILQPFVVLKSHYGTETKPSQGTTFDEK
YFERQLYVLRKQATHTLKLESGFYICSLSPKNIVYKGQLSPPQVYNYYHDLNHVLFASHF
CLVHSRFSTNTFPSWDRAQPMRWAAHNGEINTVRGNKAWMRAKEGNMKSSTFGDELELLY
PIVEAGGSDSAAFDNVLELLVVNGVLSLPEAVMVMVPESFQNNPDMEADKRAFYEWAACI
MEPWDGPALFTFSDGRYCGANLDRNGLRPCRWVTTNEDIMVCASEVGAITIEPEKVTRKG
RLQPGKMLLVDTVEGRIVDDKELKHNSAKRQPFANWIARELLRLPEIMAKEKAKGAELSV
ELDDSTVSTDPRLLAAGYSFEQLDLLLRPLVNDGKEALGSMGNDAPLACMATAPRILYDY
FRQLFAQVTNPPIDPIREALVMSLESTVGAGPNVLEMNAKQCGRLLLPSPVLTIEETNAL
KRLSIVYSDWQSRTIDITFDKAEGVQGYVRCLDRVCAEVTQAIADDIRVVILSDRNVGPS
RVAISSAVATGGVHHHLVRNKQRNKVALVVESAEAREVHHVCVLVGYGADAVNPYLCLEA
MLKLRREGLLKGDLTEQQVVDNYKHAVDNGILKVMSKMGISVLSSYKGAQVFEALGLAEE
VVERCFTGTASRIQGATFEMLALDALETHERGYPSRETVLPPGLPETGEYHWRDGGEAHI
NDPAGLASLQDAVREKNQASYDAYAANALRQVRAVTLRGLLEFEFSKGQSIPIEQVEPWH
EIVKRFVTGAMSYGSISMEAHSTLAIAMNRIGGKSNTGEGGEDAERSDYLPNGDTMRSAI
KQIASGRFGVTSNYLADADELQIKMAQGAKPGEGGELPGHKVSKSIARTRHSTAGVGLIS
PPPHHDIYSIEDLKELIYDLKCANPRARVSVKLVSEVGVGVVASGVAKARADHILVSGHD
GGTGASRWTGIKYAGLPWELGLAETHQTLVLNDLRGRVTLQTDGQIRTGRDVAIACLLGA
EEWGFATTPLIAMGCVMMRKCHLNTCPVGIATQEPELRKKFTGTPEHVINFFFYVAEDLR
GIMAKLGFRTINEMVGRSDMLKVDESLRNPKTANLDLSALLKPAWQMRPGVATYKCRAQE
HKLYLRLDNKLVDEAELSLTKGLPTRIECNVVNTDRALGTTLSNHVSKRYGEEGLPKDTI
HVYAKGSAGQSLGAFLAPGITIELEGDTNDYLGKGLSGGRLILYPPEVLTQPGSAFKSEE
NIICGNTCLYGATSGQAYIRGIAAERFAVRNSGATAVVEGCGDHGCEYMTGGRVVVLGSV
GRNFAAGMSGGIAYVFDAQKDFRSKVNMEMVELDTVNDPHEIAELRGMIEDHKHYTGSEL
ASSILRSFNQVLPRFVRVMPLDYKAVLEAEAARAAAEKKRKFAPAKPTPIVDASKAAAVP
IENGSKAAEPAVVDLEDSMVDEATAKKRIETLDKVKGFMKYKRLTEPYRNPRKRVKDWAE
LSSRLSDDECKVQTARCMDCGVPFCQSDTGCPIGNIIPKWNDLVFKGQWRDALNRLLMTN
NFPEFTGRVCPAPCEGACVLGINEQPVGIKSIECAIIDKGFEMGWMVPAPPAVRTGKRVA
IIGSGPAGLAAADQLNKAGHLVTVYERQDRFGGLLMYGIPNMKLDKRTVQRRIDLMAAEG
VQFVNKAHVGVTHDIKDIRAENDALIMATGATWPRDLKMKNRDLDGIHFAMEFLTQNTRS
LLDSNLEDGRYIDARGKDVLVIGGGDTGNDCIGTSLRHGAKSVTNFELLPQPPSARAGDN
PWPQWPKVFRVDYGHTEVAAHFGKDPREYSISTKEFISDGNGKIKGVNTVRVEWTKNALG
QWQMSEIPGSEQFFPADLIFLALGFLGPEEAAIKALGIAQDGRSNVKTPAGKYATSVEGV
FAAGDCRRGQSLVVWGINEGRSAAAEVDRFLTGDTRLPLAGSIKVRSFAPAKYTNKQPQQ
RVQVEAVAA