Protein Info for mRNA_7437 in Rhodosporidium toruloides IFO0880

Name: 15805
Annotation: K01756 purB, ADSL adenylosuccinate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 11 to 456 (446 residues), 447.6 bits, E=2.2e-138
• PF00206: Lyase_1
  • amino acids 55 to 306 (252 residues), 83.9 bits, E=1.4e-27
• PF10397: ADSL_C
  • amino acids 374 to 455 (82 residues), 56.2 bits, E=4.3e-19

Best Hits

Swiss-Prot: 72% identical to PUR8_YEAST: Adenylosuccinate lyase (ADE13) from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 81% identity to cnb:CNBG1690)

MetaCyc: 72% identical to Ade13 (Saccharomyces cerevisiae)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of histidine, purine, and pyrimidine biosynthesis (38/46 steps found)
• superpathway of purine nucleotide salvage (14/14 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (21/26 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• dZTP biosynthesis (4/5 steps found)
• inosine-5'-phosphate biosynthesis I (4/6 steps found)
• inosine-5'-phosphate biosynthesis III (3/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Protein Sequence (482 amino acids)

>mRNA_7437 K01756 purB, ADSL adenylosuccinate lyase (Rhodosporidium toruloides IFO0880)
MSPIDTYSTPLSSRYASKEMSKLFSPATRFGTWRKLWLSLATAEKQLGLSIPDEAIEQMK
ANLDLDEAQMDEAAVEEKKRRHDVMAHVHVFGLHAPAAAGIIHLGATSCYVTDNADLIFL
RDACDIILPKLAVVIERLARFAEQYKDLPTLGWTHFQPAQLTTVGKRATLWIQELLWDLR
NIQRARDDIGFRGVKGTTGTQASFLALFDGDHDKVEELDRLVTELSGFKHAYPVTGQTYS
RKIDIDVLGPLASFGATAHKIATDIRLLANLKEVEEPFEKDQIGSSAMAYKRNPMRSERI
CSLARHLMVMQQNAMMTASVQWFERTLDDSANRRITIPEAFLTADIILTTLQNVTEGLVV
YPAIIARRVRQELPFMATENIIMAIVKKGGDRQICHEKIRVLSHEAGAVVKQQGGENDLI
DRVRADKFFEPIWNDLDRLLDPSTFVGRAPEQTTKFVKEHVKPAIEPYKTAVDAAVAAEL
SV