Protein Info for mRNA_7720 in Rhodosporidium toruloides IFO0880

Name: 16088
Annotation: K03177 truB, PUS4, TRUB1 tRNA pseudouridine55 synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01509: TruB_N
  • amino acids 68 to 199 (132 residues), 141.7 bits, E=2.4e-45
• PF16198: TruB_C_2
  • amino acids 286 to 316 (31 residues), 31.1 bits, see alignment (E = 2.2e-11)

Predicted SEED Role

"tRNA pseudouridine synthase B (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70

Use Curated BLAST to search for 4.2.1.70

Protein Sequence (403 amino acids)

>mRNA_7720 K03177 truB, PUS4, TRUB1 tRNA pseudouridine55 synthase (Rhodosporidium toruloides IFO0880)
MPKAGNAPLSALFGLNKPTGVPSMTLLNRLQPLFSASELFKDPNSTDEQDQGGKGWKKGK
QGRGRRANKVKMGQGGTLDPLADGVLVVGTNAATKSLSRFLDCTKEYRAIGLLGCSTDSY
DSDGKRVKMASWQGITKEKVEEVLGRFRGEIEQTPPVYSALKMDGKPLYEYARSNTPLPR
PIPPRKVTVHSLELLRFANGDEHTYEYPKEELEEDKKVELERLEKMVKEGRTAMPSEEEV
AQGAEEAGASAPAAASSNEAGPSDSSASRPPIFEIHLTVSSGTYIRSIVHDIGLALGSVA
HVVKLTRTRQGEFVLEPPNASTVLSAGDGTEETEGDGADVVKEKTADGLELETFTGGCVE
WSLLEKAIEEQAKAKKEDREVEGRDQDGWLEWERELLRKCKRV