Protein Info for mRNA_7840 in Rhodosporidium toruloides IFO0880

Name: 16208
Annotation: K00620 argJ glutamate N-acetyltransferase / amino-acid N-acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 82 to 498 (417 residues), 468.5 bits, E=8.5e-145
• PF01960: ArgJ
  • amino acids 88 to 498 (411 residues), 510.1 bits, E=1.6e-157

Best Hits

Swiss-Prot: 64% identical to ARGJ_POSPM: Arginine biosynthesis bifunctional protein ArgJ, mitochondrial (POSPLDRAFT_134690) from Postia placenta (strain ATCC 44394 / Madison 698-R)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 64% identity to ppl:POSPLDRAFT_134690)

MetaCyc: 50% identical to glutamate N-acetyltransferase (Saccharomyces cerevisiae)
Glutamate N-acetyltransferase. [EC: 2.3.1.35]; Amino-acid N-acetyltransferase. [EC: 2.3.1.35, 2.3.1.1]

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (9/9 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• superpathway of arginine and polyamine biosynthesis (13/17 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-ornithine biosynthesis I (4/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.1, 2.3.1.35

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Protein Sequence (498 amino acids)

>mRNA_7840 K00620 argJ glutamate N-acetyltransferase / amino-acid N-acetyltransferase (Rhodosporidium toruloides IFO0880)
MAGLAKAAQAQLKQALAAPAARLFSSASAAPYPASSSQPPAPGGSGNGENGAGAANGQAK
PKLTKAQRFTLDPATASTPPKFPHGFLATGLHCGVKKDRSKLDLAVIISDRPCSAAGTFT
RNAFVAAPVIVSQEVLARQRGIARGLVVNSGCANAVTGSKGLEDARAMSDQLDSFLSASS
SSDGTGTLVMSTGVIGQLLPIEKIRQGIEASTATLGSTFSAWSDCARAFMTTDTFPKLRT
RTIQLGGKEVRMVGIDKGAGMIHPNMGPPAAPHATMLGVIATDAAVEPASLQNALTYAVD
RSFNSISVDGDMSTNDTVLVLANGASGMAEISEKKTPEEFVRFREELTSFAAELAQLVVR
DGEGAEKFVKVTVQGAPSYEGAHKIASTVSTSSLVKCALHGEDANWGRILCAVGYSQPPF
PIDPTKVTVSFVPQDGSPELKLLVNGEPENVDEERASEILREDDLEIKIDLGLGEETATY
WTCDLSHEYISINADYRS