Protein Info for mRNA_8313 in Rhodosporidium toruloides IFO0880
Name: 16681
Annotation: K11541 URA2 carbamoyl-phosphate synthase / aspartate carbamoyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K11541, carbamoyl-phosphate synthase / aspartate carbamoyltransferase [EC: 2.1.3.2 6.3.5.5] (inferred from 68% identity to afm:AFUA_6G11310)Predicted SEED Role
"Carbamoyl-phosphate synthase large chain (EC 6.3.5.5) / Carbamoyl-phosphate synthase small chain (EC 6.3.5.5) / Aspartate carbamoyltransferase (EC 2.1.3.2) / Dihydroorotase (EC 3.5.2.3)" in subsystem De Novo Pyrimidine Synthesis or Macromolecular synthesis operon (EC 2.1.3.2, EC 3.5.2.3, EC 6.3.5.5)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (38/46 steps found)
- L-arginine biosynthesis II (acetyl cycle) (9/9 steps found)
- superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (15/18 steps found)
- UMP biosynthesis II (6/6 steps found)
- L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
- urea cycle (5/5 steps found)
- superpathway of L-citrulline metabolism (10/12 steps found)
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of arginine and polyamine biosynthesis (13/17 steps found)
- UMP biosynthesis I (5/6 steps found)
- UMP biosynthesis III (5/6 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
- superpathway of pyrimidine ribonucleotides de novo biosynthesis (7/9 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-arginine biosynthesis IV (archaea) (6/9 steps found)
- L-glutamate and L-glutamine biosynthesis (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.5.2.3, 6.3.5.5
Use Curated BLAST to search for 2.1.3.2 or 3.5.2.3 or 6.3.5.5
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (2253 amino acids)
>mRNA_8313 K11541 URA2 carbamoyl-phosphate synthase / aspartate carbamoyltransferase (Rhodosporidium toruloides IFO0880) MLLELADGSAYEGYSFGADKSIAGECVFTTGMVGYPESLTDPSYSGQILVLTYPLIGSYG VPKRPAKGTVNPASLPHEFESYKIHVAGLIVSNYSGAGDDFSNHSAFSELADWLKEEGIP AIYGVDTRDLTKKLRERGSTIGRLSQRKKGVEVEEGAFNWREAYEQLEYHDPNADNLVAA VSTPQPVLYPADSGVSPLKHTSGRNLRVICVDVGMKYNQIRCFTKRGVELKVVPYDYDYL SPNEEPFDGLFVSNGPGDPATLKSTTAILAKAIEAAKVPIFGICLGHQLLARASGASTLK LKYGNRGANIPCTSLVTGRCYITSQNHGYAVDTQTLQQGWKELFINANDSSNEGIYSTER PYFSVQFHPESAPGPRDTEFLFDTFISAIQKSLEKQASGEKLTGPVVFPGGDAAENAAKT PRVDVRKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINRELERVVDARPPAA NIATIQTSKGLADKVYFLPVTADSVRKVIQHERPDGIYCTFGGQTALQVGIKLKDEFEGL GVKVLGTPIETIITTEDRELFAQAMEQIGEKCAKSAAANSVQECIDAANDIGYPVIVRAA FALGGLGSGFANNEKELVELCTKAFATSSQVLVERSMKGWKEIEYEVVRDCRDNCITVCN MENFDPLGIHTGDSIVVAPSQTLTDSDYNMLRTTAVNVIRHLGVVGECNIQYALNPHSQE YCIIEVNARLSRSSALASKATGYPLAYTAAKLGLNIPLNEIKNSVTKNTSACFEPSLDYL VVKIPRWDLKKFTRVSSSLSSSMKSVGEVMAIGKTFEETIQKAIRAIDPSFAGFGKNAFP MEEVDEELQNPTDRRLFAIANALHAGRSVEEVHKLTNIDRWFLHKLEHIVQVDKTVAQYT ASQIPRGLLHCAKRTGFSDAQIAKALGSNELAVRRLRLDYGITPRVKQIDTVAAEFPAFT NYLYMTYNGAAHDVNFGDKGIMVLGSGVYRIGSSVEFDWCAVRAIRTIRERGFKTVMVNY NPETVSTDYDEADRLYFEVISLETILDIYEAETSSGVVVSMGGQTSNNIALPLHRQGVKI LGTSPENIDMAENRYKFSRMLDKLGVDQPLWKELSTMEAAHEFCNKVGYPVLVRPSYVLS GAAMNVVYSGDDLDAYLKQAAAVSREYPVVVTKYVEEAKEIEMDAVARDGKLVMHYVSEH VENAGVHSGDATLILPPQDLDPETVRRIEEATRKIGAALNVSGPFNIQFLAKNNEIKVIE CNVRAARSFPFVSKVTGIDAIELATDIMLGLPVTPYPELNLPKDYVGIKVPQFSFGRLAG ADPILGVEMASTGEVACFGRDKYEAYLKALLSTGMRLPKQNILFSIGPYKEKIELLPSIQ KLHSLGYNIFATVGTADFLQEHGVPVKYLEALDEEDDRKAQKSEYSLREHIANNKIDLYV NLPSKNRYRRPANYMSKGYRSRRMAVDFAVPLVTNVKCAKLLIQAIARNPSLEISNVDYK TSSRTVVLPGLVNTRCFVPGVAAPGSKDFAAVTKAALAGGFTTVQVLPVGDKANVVDSPS LDVARDNAASAACDYSLAIAATADNASHLTGELIAGARTLFLPSQNLSASNDKVSTVAEH FSAWPLNKPIVTDARTTDLASVLLLASLHGRSVHVTNVQTENDISLISLSKDKDLKVTCD VSIYSLFLTREEFPGSTCLPTAKDQAALWDHLDTIDVFAVGALPFRLATELGKPYSPEAG QEEAMQLLLTAVHDGRLSLDDIISRLSENPRAIFDLPPSPTETYVEVEVDRTVVVPRRSY WSPLEGKKLAGAVHRVVLRGETLLLDGQFVARGAPAGRDLSALAGASKTERRQARFSVSA TRPSLASIGMPTRENVTSPTMAPVSSQALATKSPKLASARSPRLEATESALQPSLMSLAT DPVASSLALVSPMASLRKLLSAASRAYNRRHVLSVRSFNRDDLHTLFGVASEMRMLTERG VPIDIMRGRVLSTMFYEPSTRTSASFEAAMARLGGSTVAVTPETSSVVKGETLADTIRTL GCYADVIALRHPAAGSAQEAARYSPVPVINAGDGIGEHPTQAFLDIYTIREELGTVNGLT ITMVGDLKNGRTVHSLVKLLSLYSVSLIFVAPPTLSMPESVKAEAEKAGIPVFETTSLAS VIGRTDVLYVTRVQQERFASQAEYDAVKDSYIVNNEILSGAKEHCIVMHPLPRNAELDPS VDLDARRAAFWRQVRFGLFVRMALLALVAAGSA