Protein Info for mRNA_217 in Rhodosporidium toruloides IFO0880

Name: 8585
Annotation: K02292 crtO beta-carotene ketolase (CrtO type)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 554 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details transmembrane" amino acids 532 to 552 (21 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 13 to 55 (43 residues), 28.6 bits, see alignment 3.1e-10 TIGR02734: phytoene desaturase" amino acids 14 to 509 (496 residues), 430.6 bits, see alignment E=4.3e-133 PF00890: FAD_binding_2" amino acids 14 to 48 (35 residues), 22.2 bits, see alignment 2.4e-08 PF13450: NAD_binding_8" amino acids 16 to 71 (56 residues), 47.4 bits, see alignment 6.5e-16 PF01593: Amino_oxidase" amino acids 23 to 292 (270 residues), 71.3 bits, see alignment E=3.8e-23

Best Hits

Swiss-Prot: 54% identical to CRTI_NEUCR: Phytoene desaturase (al-1) from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

KEGG orthology group: None (inferred from 54% identity to bfu:BC1G_13907)

MetaCyc: 54% identical to phytoene desaturase (3,4-didehydrolycopene-forming) (Neurospora crassa)
RXN-12413 [EC: 1.3.99.30]

Predicted SEED Role

"Phytoene dehydrogenase (EC 1.14.99.-)" in subsystem Carotenoids (EC 1.14.99.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.99.-

Use Curated BLAST to search for 1.14.99.- or 1.3.99.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (554 amino acids)

>mRNA_217 K02292 crtO beta-carotene ketolase (CrtO type) (Rhodosporidium toruloides IFO0880)
MAAANGHGKGKPSVLIVGAGVGGTASAARLAQSGFDVTVLEKNDFAGGRCSLFTDPTKSF
RFDQGPSLFLIPRLFDETFSDLGTSLENEGIKLVKCEPNYRIVFPDKEVVEMSSDLTRMK
KQVERWEGEKGFEGFLGFLKEGHAHYELSMVHVLHRNFTSLLSMVRPSLIIQLRKLHPFV
SVYSRATKYFKTDRMRRAFTFASMYLGMSPFDALGAYNLLQYTEHCEGILYPLGGFGRIP
QTLQKLAEKSGAKFRFNSPVKRVTVENGTAKGVELESGEKLKAEIVLVNADLVWSMAHLY
EETSYSKRLEERPVSCSSISFYWSMNRKIPQLDSHTIFLAEEYRESFDSIFREHRIPHEP
SFYVNVPSRHDPSAAPADKDAVIVLVPVGHISAALPSSSDWDKVVEDTRNKIIGEIERRL
DIKDLRGCIEHETINTPITWGEKFNLHRGSILGLSHDFFNVLSFRPKTRHPSVKNAYFVG
ASAHPGTGVPIVLAGARLVATQILNDLGMPIPSRWNVSSSELATHKTIRDAAGGFTLLSV
LFGLIALLVMYLRG