Protein Info for mRNA_517 in Rhodosporidium toruloides IFO0880

Name: 8885
Annotation: K07508 ACAA2 acetyl-CoA acyltransferase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00108: Thiolase_N
  • amino acids 4 to 267 (264 residues), 259.5 bits, E=4.9e-81
• TIGR01930: acetyl-CoA C-acyltransferase
  • amino acids 6 to 395 (390 residues), 421.6 bits, E=1.4e-130
• PF02803: Thiolase_C
  • amino acids 275 to 396 (122 residues), 152 bits, E=9e-49

Best Hits

Swiss-Prot: 57% identical to THIM_HUMAN: 3-ketoacyl-CoA thiolase, mitochondrial (ACAA2) from Homo sapiens

KEGG orthology group: K07508, acetyl-CoA acyltransferase 2 [EC: 2.3.1.16] (inferred from 64% identity to mgl:MGL_1340)

MetaCyc: 57% identical to 3-ketoacyl-CoA thiolase, mitochondrial (Homo sapiens)
Acetyl-CoA C-acyltransferase. [EC: 2.3.1.16]; 2.3.1.16 [EC: 2.3.1.16]

Predicted SEED Role

"3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9)" (EC 2.3.1.16, EC 2.3.1.9)

MetaCyc Pathways

• superpathway of glyoxylate cycle and fatty acid degradation (13/14 steps found)
• superpathway of geranylgeranyldiphosphate biosynthesis I (via mevalonate) (10/10 steps found)
• mevalonate pathway I (eukaryotes and bacteria) (7/7 steps found)
• isoprene biosynthesis II (engineered) (7/8 steps found)
• oleate β-oxidation (26/35 steps found)
• fatty acid salvage (5/6 steps found)
• valproate β-oxidation (7/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (4/5 steps found)
• mevalonate pathway II (haloarchaea) (5/7 steps found)
• ketolysis (2/3 steps found)
• L-isoleucine degradation I (4/6 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• acetoacetate degradation (to acetyl CoA) (1/2 steps found)
• glycerol degradation to butanol (11/16 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• ketogenesis (3/5 steps found)
• mevalonate pathway IV (archaea) (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• polyhydroxybutanoate biosynthesis (1/3 steps found)
• propanoate fermentation to 2-methylbutanoate (3/6 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (2/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• fatty acid β-oxidation VII (yeast peroxisome) (2/5 steps found)
• mevalonate pathway III (Thermoplasma) (4/8 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• fatty acid β-oxidation I (generic) (3/7 steps found)
• pyruvate fermentation to butanoate (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (4/9 steps found)
• isopropanol biosynthesis (engineered) (1/5 steps found)
• pyruvate fermentation to acetone (1/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (16/26 steps found)
• superpathway of ergosterol biosynthesis I (16/26 steps found)
• acetyl-CoA fermentation to butanoate (2/7 steps found)
• 10-trans-heptadecenoyl-CoA degradation (MFE-dependent, yeast) (1/6 steps found)
• 4-ethylphenol degradation (anaerobic) (1/6 steps found)
• 2-deoxy-D-ribose degradation II (2/8 steps found)
• 2-methylpropene degradation (2/8 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (4/11 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (3/10 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
• L-tryptophan degradation III (eukaryotic) (6/15 steps found)
• L-lysine fermentation to acetate and butanoate (2/10 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (4/13 steps found)
• 4-oxopentanoate degradation (1/9 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (3/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (3/12 steps found)
• L-glutamate degradation VII (to butanoate) (3/12 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (4/14 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (6/17 steps found)
• jasmonic acid biosynthesis (7/19 steps found)
• ethylmalonyl-CoA pathway (1/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (5/18 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• sitosterol degradation to androstenedione (2/18 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (4/22 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• superpathway of cholesterol biosynthesis (14/38 steps found)
• superpathway of testosterone and androsterone degradation (6/28 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (26/57 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of L-lysine degradation (11/43 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (10/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Ethylbenzene degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Synthesis and degradation of ketone bodies
• Terpenoid biosynthesis
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.16, 2.3.1.9

Use Curated BLAST to search for 2.3.1.16 or 2.3.1.9

Protein Sequence (397 amino acids)

>mRNA_517 K07508 ACAA2 acetyl-CoA acyltransferase 2 (Rhodosporidium toruloides IFO0880)
MSAVYIASAKRTAFGAFGGALKSYTASQLGGFAAKAALAELPEGTKVDSVIFGQVLYSDP
SAAYLARHVGHLAGLPVDVPALTVNRLCGSGFQSIFNAAQEIRLGESHVVLTGGTDNMSL
SPYTLSGASRFGTKYGVDLKLEDSLAQALTDRVPNPTPMGITAENLANQYGITREDCDKY
AQQSQQRWKQGLDSGAFEGEIAPVTLPPKKKGGEPIVFKQDEHPRPSVTLEQLAKLPPVF
AKNGTVTAGNASGICDGAAANVVVSEEAIKRYGIKPLARVVAYHVNAVEPTVMGIGPVDG
IRGVLKKAGLKIEDIDLFDINEAFAAQWLAVQKELGLSMDKSNVHGGAIALGHPLAASGA
RITNHLVHNLHRLNKRYAVGSACIGGGQACSIVLERV