Protein Info for mRNA_579 in Rhodosporidium toruloides IFO0880
Name: 8947
Annotation: K10686 UBE1C, UBA3 ubiquitin-activating enzyme E1 C
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 54% identical to UBA3_DICDI: NEDD8-activating enzyme E1 catalytic subunit (uba3) from Dictyostelium discoideum
KEGG orthology group: K10686, ubiquitin-activating enzyme E1 C [EC: 6.3.2.19] (inferred from 72% identity to scm:SCHCODRAFT_65491)MetaCyc: 52% identical to NEDD8-activating enzyme E1 catalytic subunit (Homo sapiens)
RXN-18742 [EC: 6.2.1.64]; UBIQUITIN--PROTEIN-LIGASE-RXN [EC: 6.2.1.64, 6.2.1.45]
Predicted SEED Role
"Sulfur carrier protein adenylyltransferase ThiF" in subsystem Thiamin biosynthesis
MetaCyc Pathways
- protein NEDDylation (3/3 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (4/7 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (3/7 steps found)
- superpathway of thiamine diphosphate biosynthesis II (5/11 steps found)
- protein ubiquitination (3/9 steps found)
- superpathway of thiamine diphosphate biosynthesis I (4/11 steps found)
Isozymes
Compare fitness of predicted isozymes for: 6.3.2.19
Use Curated BLAST to search for 6.2.1.45 or 6.2.1.64 or 6.3.2.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (426 amino acids)
>mRNA_579 K10686 UBE1C, UBA3 ubiquitin-activating enzyme E1 C (Rhodosporidium toruloides IFO0880) MVASKYAHLDRVLTRAGPYVDPESFQGGAEVQSFLRDQCKVLVIGAGGLGCEILANLALM GFADIHVIDMDTIDVSNLNRQFLFRWVSRRPEDVGKSKAQCAADFVMKRVPGCKVTPYHG KIQDKDDDYYMQFNIVICGLDSVDARRWISATLVNLVDPEVPESLKPLIDGGTEGFKGQA RVILPTITSCYECSLDMLTPPTAFPICTIANTPRQPEHCIEWASILEWPKVFKDTKLDND DPEHIQWLFDTARKRAEEFNIPGVTWSLTQGVVKRIIPAIASTNAIVSAACCNEAFKIAT STNPYLNNYMMYTGNESIYTYTFEHQRKPECPVCGGEKVTVSQKPSNSLQDLIDMLLERQ EFQIRRPSLRLASKSLYLQAPPQLEEATRPNLEKTLAELMQSGDVVTVTDAGLPFSLDLV VQFEQE