Protein Info for mRNA_745 in Rhodosporidium toruloides IFO0880

Name: 9113
Annotation: K00101 E1.1.2.3, lldD L-lactate dehydrogenase (cytochrome)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 transmembrane" amino acids 41 to 61 (21 residues), see Phobius details PF00173: Cyt-b5" amino acids 86 to 158 (73 residues), 70.8 bits, see alignment E=1.8e-23 PF01070: FMN_dh" amino acids 200 to 551 (352 residues), 378.9 bits, see alignment E=4.9e-117

Best Hits

Swiss-Prot: 73% identical to CYBL_RHOGR: (S)-mandelate dehydrogenase, mitochondrial from Rhodotorula graminis

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (561 amino acids)

>mRNA_745 K00101 E1.1.2.3, lldD L-lactate dehydrogenase (cytochrome) (Rhodosporidium toruloides IFO0880)
MAFARARTVARNLHQLLNRRPLSTASPSQGTAARAASKRAFQLGVGAGAVLTVGGVAAYL
YTQSPVHLDAPSPLPAKQRRRDRSLTAAEVAKHNSKGDIWVVIDSQVWDISDFAEIHPGG
IKVLEQNAGKDVTKVFKTIHPPKTLEKFLTDDNLVGYIDVDEATKIGGGKNAEDFRIEQA
RESLRNVDTIVCLDEFEEIAQSILTEMAASYYSTGSETEQSLRDERESWQRIRFRPRVMR
KMRHIDSRTSFLGMPQPLPFFIAPAGLARLGHPDGELNITRGAAEHDVVQIVSSGASASI
DEIFGEKQPDQTLFWQLYLPSDREAGAKKIKHAVELGAKAIFVTADVPVLGKRERDLKLK
ARSQSYEHPIAAQWKAAGASDWDAVKQRGVSDIPDTAHIDANLCWEDIPWIRKQAPGIPI
VVKGVGCVEDVEEAKRHGADGVVLSTHGGRQLDGARAPIDVLVEIRKKNPELLKEIEVYI
DGGARRGTDVVKALCLGAKGVGFGRSFLYAQSAFGAAGVSKAIKILEEEVHSTMRLLGAN
TIADLKPEMVEVSSPERWVPV