Protein Info for mRNA_799 in Rhodosporidium toruloides IFO0880

Name: 9167
Annotation: K01893 NARS, asnS asparaginyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 TIGR00457: asparagine--tRNA ligase" amino acids 147 to 586 (440 residues), 424.9 bits, see alignment E=1.9e-131 PF01336: tRNA_anti-codon" amino acids 158 to 220 (63 residues), 41.8 bits, see alignment E=8.6e-15 PF00152: tRNA-synt_2" amino acids 261 to 582 (322 residues), 296.1 bits, see alignment E=3e-92

Best Hits

Swiss-Prot: 62% identical to SYNC_SCHPO: Probable asparagine--tRNA ligase, cytoplasmic (nrs1) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01893, asparaginyl-tRNA synthetase [EC: 6.1.1.22] (inferred from 63% identity to uma:UM04442.1)

Predicted SEED Role

"Asparaginyl-tRNA synthetase (EC 6.1.1.22)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.22

Use Curated BLAST to search for 6.1.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (587 amino acids)

>mRNA_799 K01893 NARS, asnS asparaginyl-tRNA synthetase (Rhodosporidium toruloides IFO0880)
MAGVIGKAQEQVAQALEAATAALSLDAVYVDEATGDDKDGDGSQAKPFKTVAGAYIARSS
DAINVHVRQAPAEGQTEGSWAAATASAKKKAAKLLQTHKNKLAKQEELRKREEAEGEARR
AAEAKKLEEAKAIVLEEPTTEATRIKIRQATEQRGKRVRVFGWVHRLRQQGGMTFVILRD
GSGYLQCVLSGRLSQTYDALTLTLESTIQITGVINQLPEGKTAPENHELTADWFAVVGKA
PGGDDAFLNQVNEDADPSQLADARHLVIRGENASAILKVRAALLQSFRQSYANLGLLEVT
PPCMVQTQVEGGATLFAFDYYGEQAYLTQSSQLYLETCLPSLGDVFCVQESFRAEKSHTR
RHLSEYTHVEAELAFLTFKDLLEHIEELICQTIDRVLADPKIKELIDHLWAGKGKFEPPS
RPFMRMDYRDAIKWLNDHNITRPDDNGVEGPHVVGDDIAEAAERKMTDTIGRPIFLINFP
KDIKSFYMSRVPGDEEYTESVDLLMPNVGEIVGGSMRISDLDELLAAYKREGIDPEPYYW
FTDQRKYGTSPHGGYGLGLERLLAWLLNRYTVRECSLYPRWTGRCTP