Protein Info for mRNA_872 in Rhodosporidium toruloides IFO0880

Name: 9240
Annotation: K01852 LSS, ERG7 lanosterol synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 627 TIGR01787: squalene/oxidosqualene cyclases" amino acids 1 to 617 (617 residues), 654.3 bits, see alignment E=1.1e-200 PF13249: SQHop_cyclase_N" amino acids 16 to 275 (260 residues), 125.4 bits, see alignment E=5.2e-40 PF00432: Prenyltrans" amino acids 25 to 68 (44 residues), 7.9 bits, see alignment 0.00058 amino acids 392 to 404 (13 residues), 7.5 bits, see alignment (E = 0.00075) amino acids 463 to 502 (40 residues), 39 bits, see alignment 1e-13 amino acids 510 to 555 (46 residues), 43.1 bits, see alignment 5.5e-15 amino acids 572 to 586 (15 residues), 9.3 bits, see alignment (E = 0.0002) PF13243: SQHop_cyclase_C" amino acids 287 to 617 (331 residues), 155.8 bits, see alignment E=3e-49

Best Hits

Swiss-Prot: 57% identical to ERG7_PNECA: Lanosterol synthase (ERG7) from Pneumocystis carinii

KEGG orthology group: K01852, lanosterol synthase [EC: 5.4.99.7] (inferred from 57% identity to lbc:LACBIDRAFT_185468)

MetaCyc: 49% identical to lanosterol synthase (Homo sapiens)
Lanosterol synthase. [EC: 5.4.99.7]

Predicted SEED Role

"Squalene--hopene cyclase (EC 5.4.99.17)" in subsystem Carotenoids (EC 5.4.99.17)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.17 or 5.4.99.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (627 amino acids)

>mRNA_872 K01852 LSS, ERG7 lanosterol synthase (Rhodosporidium toruloides IFO0880)
MFLLPGIIIAMYVTKTPIPEEWKIEIARYLANMQRDGGEDDQGWGIHFEARSSVFGTGLN
YVVLRLLGVGPDEPMMVKARNCLHKLGGCEGIPSWGKFWLAILNVHSWRGLNPTPAELWL
LPDIAPIHPWRWWIHTRNVYIPMGYLSGKGFQAEEDDLIRSLRNELYTQLYSSIDWPSLR
NSISPYDVYAPHSRVANACFAVLDVYDRLAPSWIRKKALERAYKLVVMEDENTSYQTIGP
VSKAMNMLCRWLEEGPESEAFQQHLLNIRDFMWMSKDGMMMTGTNGSQLWDASFIAQALY
ESGLASLPENQASTSLLLDWLDQCQIRDNPPRFEEAYRHRTKGAWPFSTAEQSYTVSDCT
AEGMKSVLLLQELPHITERVSYPRFCDAVDTILSLQNPSGGFASYELVRGSPLLELLNPA
EVFANIMIEYPYVECTTACVTALSVFRKKYPEYRAGEIESASKKAIDWIKTAQRADGSWY
GSWGICFTYATMFSIESLALAGEKYSNSSHVKKACEFLLGKQMEDGGWGESYKSCETEQY
VHNAKSQVVNTAWAVITLLTAKYPDPEPIRRGCRLIMSRQQPDGSWPQESIEGVFNKNAA
ISYPNFKFAWTINALGQAWKKLPTEGW