Protein Info for mRNA_876 in Rhodosporidium toruloides IFO0880

Name: 9244
Annotation: K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 599 PF02219: MTHFR" amino acids 7 to 294 (288 residues), 379.1 bits, see alignment E=7.3e-118 TIGR00677: methylenetetrahydrofolate reductase" amino acids 18 to 297 (280 residues), 393 bits, see alignment E=3.6e-122

Best Hits

KEGG orthology group: K00297, methylenetetrahydrofolate reductase (NADPH) [EC: 1.5.1.20] (inferred from 59% identity to scm:SCHCODRAFT_67475)

Predicted SEED Role

"5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20)" in subsystem Methionine Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 1.5.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.5.1.20

Use Curated BLAST to search for 1.5.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (599 amino acids)

>mRNA_876 K00297 metF, MTHFR methylenetetrahydrofolate reductase (NADPH) (Rhodosporidium toruloides IFO0880)
MVKISDKIKKAEREGRPWWSFEFFPPKTPEGWVNLYDRIERMQQLGPIFVDITWGAGGST
SEATTNFVKTAHSELGLETCMHLTCTNMPVEMVDKALKATYDSGCRNILALRGDPPRGVE
EWKPTEGGFNHAIDLVKHIRKNYGDYFCIGVAGFPEGHPQSESPEAEIRHFKKKVDAGAD
IVFTQMFYDAEVFIDWGRRLRAAGITIPIVPGIMPIQTFAAFKRRTDFAGTIVPKELWDL
LEPIKDDDAKVREVGTKYVADMCRKILNADLGIHGIHCYTMNLSRGTEMLLEEMQFVPTA
DRVKPLPWRLSLTQKRRAETTRPIFWSNRQKSYITRTRDWDEFPNGRWGDASSPAFGDVD
ALLLALPHKPQDAIKLWGKPHSLGNITALFARFCRGDLKSLPWSDQPAAKETTRIAEQLA
RINELGFLTINSQPRVDGAPSEDPAVGWGPIRGYVYQKAYLEFFCPPELVEPLLELLGDT
PSVTYHAVNKQGDFRSNTAPGPNAVTWGVFPGAEVIQPTVVDSTAFQAWKDEAYELGWQW
AQLYKETAPETYEVISRIFSEFHLVNIVFNDYRNTDEDAIFKPFFELAHRKGLSVANGH