Protein Info for mRNA_932 in Rhodosporidium toruloides IFO0880

Name: 9300
Annotation: K01803 TPI, tpiA triosephosphate isomerase (TIM)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00121: TIM
  • amino acids 6 to 246 (241 residues), 304.9 bits, E=1.9e-95
• TIGR00419: triose-phosphate isomerase
  • amino acids 6 to 241 (236 residues), 246.9 bits, E=1.1e-77

Best Hits

Swiss-Prot: 61% identical to TPIS_EMENI: Triosephosphate isomerase (tpiA) from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139)

KEGG orthology group: K01803, triosephosphate isomerase (TIM) [EC: 5.3.1.1] (inferred from 61% identity to ani:AN6900.2)

MetaCyc: 55% identical to cytosolic triose-phosphate isomerase (Arabidopsis thaliana col)
Triose-phosphate isomerase. [EC: 5.3.1.1]

Predicted SEED Role

"Triosephosphate isomerase (EC 5.3.1.1)" in subsystem Calvin-Benson cycle or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or MLST (EC 5.3.1.1)

MetaCyc Pathways

• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (20/22 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/25 steps found)
• formaldehyde assimilation III (dihydroxyacetone cycle) (11/12 steps found)
• glycolysis III (from glucose) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (10/12 steps found)
• homolactic fermentation (10/12 steps found)
• glycolysis II (from fructose 6-phosphate) (9/11 steps found)
• glycolysis IV (8/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/16 steps found)
• gluconeogenesis III (9/12 steps found)
• superpathway of anaerobic sucrose degradation (14/19 steps found)
• Calvin-Benson-Bassham cycle (9/13 steps found)
• glycerol degradation to butanol (11/16 steps found)
• glycolysis V (Pyrococcus) (6/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (18/27 steps found)
• sucrose degradation V (sucrose α-glucosidase) (2/5 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• oxygenic photosynthesis (10/17 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (11/19 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (16/26 steps found)
• superpathway of N-acetylneuraminate degradation (11/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/21 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (26/57 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Fructose and mannose metabolism
• Glycolysis / Gluconeogenesis
• Inositol phosphate metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.3.1.1

Use Curated BLAST to search for 5.3.1.1

Protein Sequence (259 amino acids)

>mRNA_932 K01803 TPI, tpiA triosephosphate isomerase (TIM) (Rhodosporidium toruloides IFO0880)
MTRKLFVGGNFKMNGDLATLTKLVHQFNEADVDVESAEVVIAPPALYLLPLKELARKGIS
VAAQNAHQLPAGAYTGEISLQQLKDIGLNWVILGHSERRTIFHESDELVAEKTAAAIKTG
VSVIFCIGETLEEREANKTEEVVTRQTDALAKVIAEEDWKHIVVAYEPVWAIGTGKVATP
QQAQDTHKVIRDFIAKRVSQKVAEDTRIIYGGSVSGKNSKELCFQPDIDGALVGGASLKP
EFVDVIRAAEEAYKSKAKL