Protein Info for mRNA_1299 in Rhodosporidium toruloides IFO0880

Name: 9667
Annotation: K00600 glyA, SHMT glycine hydroxymethyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00464: SHMT
  • amino acids 18 to 405 (388 residues), 586.3 bits, E=2.4e-180

Best Hits

Swiss-Prot: 62% identical to GLYC2_DICDI: Serine hydroxymethyltransferase 2 (shmt2) from Dictyostelium discoideum

KEGG orthology group: K00600, glycine hydroxymethyltransferase [EC: 2.1.2.1] (inferred from 73% identity to cne:CNJ00410)

MetaCyc: 56% identical to serine hydroxymethyltransferase, cytosolic (Homo sapiens)
Glycine hydroxymethyltransferase. [EC: 2.1.2.1]; RXN-9896 [EC: 2.1.2.1]

Predicted SEED Role

"Serine hydroxymethyltransferase (EC 2.1.2.1)" in subsystem Folate Biosynthesis or Glycine Biosynthesis or Glycine and Serine Utilization or LMPTP YwlE cluster or Photorespiration (oxidative C2 cycle) or Serine-glyoxylate cycle or Serine Biosynthesis (EC 2.1.2.1)

MetaCyc Pathways

• folate polyglutamylation (5/5 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• glycine biosynthesis I (1/1 steps found)
• superpathway of L-serine and glycine biosynthesis I (3/4 steps found)
• folate transformations II (plants) (8/11 steps found)
• dTMP de novo biosynthesis (mitochondrial) (2/3 steps found)
• folate transformations I (9/13 steps found)
• photorespiration (6/9 steps found)
• L-carnitine biosynthesis (2/4 steps found)
• glycine betaine degradation I (4/7 steps found)
• glycine betaine degradation II (mammalian) (1/4 steps found)
• purine nucleobases degradation II (anaerobic) (14/24 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Glycine, serine and threonine metabolism
• Methane metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.1

Use Curated BLAST to search for 2.1.2.1

Protein Sequence (471 amino acids)

>mRNA_1299 K00600 glyA, SHMT glycine hydroxymethyltransferase (Rhodosporidium toruloides IFO0880)
MASIPIPNDFNKCLYTPLAEADPEIQALVDRETYRQFRGLELIASENLTSLAVMQANGSV
FTNKYSEGLPGARYYGGNEVVDELEVLCQNRALEAFHLDGAKWGVNVQPYSGSTANFAAF
TALIQPQDRIMGLNLADGGHLTHGAYTRKRKISASSIYFQPLPYQVDHSTGIIDYDALEK
NAILFKPRILICGASAYPRDWDYARLRKIADINGSYLMMDMAHISGLVAAQVQNDPFEHC
DVVCTTTHKTLRGPRAGLIFFRKDKEADLEKRINDAVFPACQGGPHNNTIAAVAVALKQA
ATPEFKEYSTQIIKNTRTIAEELVKHDYKLQTEGSDNHLVLWDLRPLGLTGSKIEKLCDL
AEITLNKNSVPGDKSAATPGGVRIGSNALTSRSMKEDEMRQVADFLHRAVQIALAAQKEA
GSKLIKDFERVLKGDGEAAKQLKQLSEDVQAFATKFPLPGVPDSSTIKKTH