Experiment setAFACS_All for Rhodosporidium toruloides IFO0880

Compare to:

Sorted No BODIPY Gate

Quality Metrics:

Time0 1/1/16 setA which Time0s the sample was compared to
cor12 0.09 rank correlation(fit1, fit2), where fit1 is fitness for the first half (10-50%) and fit2 is fitness for the second half (50-90%) of each gene
maxFit 4.55 The maximum fitness value
opcor -0.02 rank correlation(upstream gene, downstream gene) over pairs that are adjacent and likely to be in the same operon
adjcor -0.00 like opcor but for adjacent genes that are not on the same strand
gccor -0.03 linear correlation of gene fitness and gene GC content
mad12 0.49 median absolute difference of fit1, fit2
mad12c 1.08 median absolute difference of log count for 1st and 2nd half of genes in this sample
mad12c_t0 1.01 like mad12c but for the Time0s
gMed 170 median reads per gene in this sample
gMedt0 164 median reads per gene in the Time0 sample
gMean 284 mean reads per gene in this sample
nMapped 16.959 M #reads for this sample that corresponded to a known strain (in millions)
nPastEnd 0.000 M #reads that corresponded to a strain that has an insertion within the suicide vector instead of within the genome.
nGenic 6.363 M #reads that lie within central 10-90% of a gene
nUsed 6.227 M #reads used to estimate gene fitness (genic and enough coverage for strain and for gene)

Specific Phenotypes

None in this experiment

For Rhodosporidium toruloides IFO0880 in facs on lipid accumulation experiments

For facs on lipid accumulation CN120 across organisms