Experiment setAFACS_Bot for Rhodosporidium toruloides IFO0880

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Sorted Low BODIPY Gate

200 most detrimental genes:

  gene name fitness t score description  
mRNA_1448 9816 +3.6 16.3 KOG4159 Predicted E3 ubiquitin ligase compare
mRNA_8013 16381 +2.8 10.7 SUPERFAMILY--SSF58113 compare
mRNA_7116 15484 +2.7 17.8 K04393 CDC42 cell division control protein 42 compare
mRNA_1657 10025 +2.7 26.3 K08286 E2.7.11.- protein-serine/threonine kinase compare
mRNA_4052 12420 +2.6 9.4 HMMPfam-Transcription factor Opi1-PF08618 compare
mRNA_628 8996 +2.5 8.4 K17894 GATA2 GATA-binding protein 2 compare
mRNA_2675 11043 +2.4 10.4 HMMPfam-Putative adipose-regulatory protein (Seipin)-PF06775 compare
mRNA_8092 16460 +2.3 12.0 K14457 MOGAT2, MGAT2 2-acylglycerol O-acyltransferase 2 compare
mRNA_224 8592 +2.3 1.4 K07300 chaA, CAX Ca2+-H+ antiporter compare
mRNA_3556 11924 +2.1 12.8 KOG0254 Predicted transporter (major facilitator superfamily) compare
mRNA_5084 13452 +2.1 9.9 Hypothetical Protein compare
mRNA_3523 11891 +2.0 6.0 K08064 NFYA nuclear transcription factor Y, alpha compare
mRNA_3244 11612 +2.0 2.2 Hypothetical Protein compare
mRNA_5620 13988 +2.0 0.8 HMMPfam-GRF zinc finger-PF06839 compare
mRNA_2688 11056 +1.9 2.7 Hypothetical Protein compare
mRNA_2891 11259 +1.9 4.8 Hypothetical Protein compare
mRNA_4302 12670 +1.8 10.4 K07819 B3GALT1 beta-1,3-galactosyltransferase 1 compare
mRNA_7273 15641 +1.8 8.9 K15859 SKN7 osomolarity two-component system, response regulator SKN7 compare
mRNA_7153 15521 +1.8 8.8 Hypothetical Protein compare
mRNA_3254 11622 +1.8 2.6 K12161 URM1 ubiquitin related modifier 1 compare
mRNA_2699 11067 +1.8 3.6 K08066 NFYC nuclear transcription factor Y, gamma compare
mRNA_2063 10431 +1.8 9.9 SUPERFAMILY--SSF140860 compare
mRNA_658 9026 +1.8 11.3 K11842 USP12_46 ubiquitin carboxyl-terminal hydrolase 12/46 compare
mRNA_6212 14580 +1.8 1.3 SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045 compare
mRNA_4290 12658 +1.7 1.9 Hypothetical Protein compare
mRNA_7113 15481 +1.7 2.3 Hypothetical Protein compare
mRNA_4725 13093 +1.7 2.6 Hypothetical Protein compare
mRNA_5725 14093 +1.7 0.5 K03234 EEF2 elongation factor 2 compare
mRNA_3955 12323 +1.6 1.2 K02355 fusA, GFM, EFG elongation factor G compare
mRNA_2885 11253 +1.6 11.2 K20246 EGT1 L-histidine Nalpha-methyltransferase / hercynylcysteine S-oxide synthase compare
mRNA_274 8642 +1.6 1.7 BLAST Putative Pc16g06240 protein [Torrubiella hemipterigena] compare
mRNA_5098 13466 +1.6 3.1 K08065 NFYB nuclear transcription Y subunit beta compare
mRNA_3656 12024 +1.6 2.1 Hypothetical Protein compare
mRNA_4541 12909 +1.6 6.0 K03457 TC.NCS1 nucleobase-cation symporter-1, NCS1 family compare
mRNA_8213 16581 +1.5 1.7 KOG1590 Uncharacterized conserved protein compare
mRNA_5173 13541 +1.5 2.6 KOG1189 Global transcriptional regulator, cell division control protein compare
mRNA_5246 13614 +1.5 3.5 K00411 UQCRFS1, RIP1, petA ubiquinol-cytochrome c reductase iron-sulfur subunit compare
mRNA_8155 16523 +1.5 5.0 K15361 WDR48, UAF1 WD repeat-containing protein 48 compare
mRNA_4564 12932 +1.4 7.6 K11844 USP16_45 ubiquitin carboxyl-terminal hydrolase 16/45 compare
mRNA_2521 10889 +1.4 1.0 BLAST beta-glucosidase 1 precursor [Aspergillus rambellii] compare
mRNA_5142 13510 +1.4 4.8 K13577 SLC25A10, DIC solute carrier family 25 (mitochondrial dicarboxylate transporter), member 10 compare
mRNA_2973 11341 +1.4 11.8 K11996 MOCS3, UBA4 adenylyltransferase and sulfurtransferase compare
mRNA_3786 12154 +1.4 4.9 K00006 GPD1 glycerol-3-phosphate dehydrogenase (NAD+) compare
mRNA_8007 16375 +1.4 6.9 KOG0590 Checkpoint kinase and related serine/threonine protein kinases compare
mRNA_3585 11953 +1.3 1.5 KOG1339 Aspartyl protease compare
mRNA_4449 12817 +1.3 9.1 K14168 CTU1, NCS6 cytoplasmic tRNA 2-thiolation protein 1 compare
mRNA_2396 10764 +1.3 8.7 K14169 CTU2, NCS2 cytoplasmic tRNA 2-thiolation protein 2 compare
mRNA_5552 13920 +1.3 1.5 K03360 GRR1 F-box and leucine-rich repeat protein GRR1 compare
mRNA_5108 13476 +1.3 3.5 BLAST V175_0103_1006-like protein [Amanita sp. 'sp-jack1'] compare
mRNA_7764 16132 +1.3 3.0 Hypothetical Protein compare
mRNA_7152 15520 +1.2 3.8 Hypothetical Protein compare
mRNA_1033 9401 +1.2 1.8 KOG4653 Uncharacterized conserved protein compare
mRNA_8095 16463 +1.2 7.3 K17508 PTC7, PPTC7 protein phosphatase PTC7 compare
mRNA_7063 15431 +1.2 1.6 Hypothetical Protein compare
mRNA_5732 14100 +1.2 3.3 HMMPfam-bZIP transcription factor-PF00170,HMMPfam-Minimal binding motif of Hap4 for binding to Hap2/3/5-PF10297,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,SUPERFAMILY--SSF57959 compare
mRNA_7315 15683 +1.2 1.9 K05527 bolA BolA protein compare
mRNA_6308 14676 +1.2 3.3 K09326 OTX1 homeobox protein OTX1 compare
mRNA_5071 13439 +1.2 8.1 KOG3038 Histone acetyltransferase SAGA associated factor SGF29 compare
mRNA_6528 14896 +1.2 1.8 K01514 PRUNE, PPX1 exopolyphosphatase compare
mRNA_7125 15493 +1.2 4.1 K11315 TADA3, ADA3, NGG1 transcriptional adapter 3 compare
mRNA_1151 9519 +1.2 1.0 Hypothetical Protein compare
mRNA_3239 11607 +1.2 5.5 K15425 PPP4R2 serine/threonine-protein phosphatase 4 regulatory subunit 2 compare
mRNA_7386 15754 +1.2 3.8 HMMPfam-F-box-like-PF12937 compare
mRNA_3533 11901 +1.2 4.9 K08337 ATG7 ubiquitin-like modifier-activating enzyme ATG7 compare
mRNA_7869 16237 +1.1 1.7 KOG4366 Predicted thioesterase compare
mRNA_4254 12622 +1.1 2.3 HMMPfam-Lactonase, 7-bladed beta-propeller-PF10282,SUPERFAMILY--SSF75011 compare
mRNA_3948 12316 +1.1 3.9 Hypothetical Protein compare
mRNA_5729 14097 +1.1 1.7 HMMPfam-MT-A70-PF05063,ProSiteProfiles-MT-A70-like family profile.-PS51143 compare
mRNA_2241 10609 +1.1 5.3 K12572 PAN3 PAB-dependent poly(A)-specific ribonuclease subunit 3 compare
mRNA_4005 12373 +1.1 0.9 HMMPfam-bZIP transcription factor-PF00170,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959 compare
mRNA_4618 12986 +1.1 1.0 Hypothetical Protein compare
mRNA_2089 10457 +1.1 4.4 KOG0637 Sucrose transporter and related proteins compare
mRNA_3677 12045 +1.1 4.9 KOG0266 WD40 repeat-containing protein compare
mRNA_1627 9995 +1.1 1.0 K18551 SDT1 pyrimidine and pyridine-specific 5'-nucleotidase compare
mRNA_4741 13109 +1.1 8.8 KOG2208 Vigilin compare
mRNA_3398 11766 +1.1 6.7 Hypothetical Protein compare
mRNA_4992 13360 +1.1 2.5 Hypothetical Protein compare
mRNA_7003 15371 +1.1 2.3 KOG2394 WD40 protein DMR-N9 compare
mRNA_2185 10553 +1.1 2.0 HMMPfam-Copper fist DNA binding domain-PF00649,PRINTS-Copper fist domain signature-PR00617,ProSiteProfiles-Copper-fist DNA-binding domain profile.-PS50073,SMART-Copper-Fist-SM00412,SMART--SM01090,SUPERFAMILY--SSF57879 compare
mRNA_2097 10465 +1.1 1.0 K03217 yidC, spoIIIJ, OXA1, ccfA YidC/Oxa1 family membrane protein insertase compare
mRNA_4660 13028 +1.0 1.5 Interpro Transcription-silencing protein, cryptic loci regulator Clr2 compare
mRNA_4524 12892 +1.0 2.7 Hypothetical Protein compare
mRNA_1806 10174 +1.0 2.6 BLAST related to C2H2 zinc finger protein [Ustilago hordei] compare
mRNA_1122 9490 +1.0 6.8 BLAST glycoside hydrolase family 28 protein [Tulasnella cal... compare
mRNA_3203 11571 +1.0 1.1 Hypothetical Protein compare
mRNA_5093 13461 +1.0 1.7 Hypothetical Protein compare
mRNA_1215 9583 +1.0 1.0 Hypothetical Protein compare
mRNA_640 9008 +1.0 2.2 HMMPfam-UV radiation resistance protein and autophagy-related subunit 14-PF10186 compare
mRNA_7106 15474 +1.0 1.3 K11313 SUPT3H, SPT3 transcription initiation protein SPT3 compare
mRNA_6139 14507 +1.0 2.3 Hypothetical Protein compare
mRNA_7790 16158 +1.0 1.0 Hypothetical Protein compare
mRNA_5329 13697 +1.0 2.8 K02212 MCM4, CDC54 DNA replication licensing factor MCM4 compare
mRNA_5978 14346 +1.0 1.8 Hypothetical Protein compare
mRNA_4405 12773 +1.0 4.9 K05925 METTL3_14 mRNA (2'-O-methyladenosine-N6-)-methyltransferase compare
mRNA_2949 11317 +1.0 3.4 K02267 COX6B cytochrome c oxidase subunit 6b compare
mRNA_1978 10346 +1.0 8.1 K14409 SMG7, EST1C protein SMG7 compare
mRNA_2644 11012 +1.0 0.8 SUPERFAMILY--SSF52058 compare
mRNA_5615 13983 +1.0 2.6 HMMPfam-GRF zinc finger-PF06839 compare
mRNA_403 8771 +1.0 7.0 Hypothetical Protein compare
mRNA_7884 16252 +1.0 2.3 KOG1911 Heterochromatin-associated protein HP1 and related CHROMO domain proteins compare
mRNA_573 8941 +1.0 1.6 BLAST proteophosphoglycan 5 [Moniliophthora roreri MCA ... compare
mRNA_4460 12828 +1.0 3.1 SUPERFAMILY--SSF52047 compare
mRNA_2074 10442 +1.0 1.0 HMMPfam-Chromate transporter-PF02417 compare
mRNA_5496 13864 +1.0 1.4 HMMPfam-HSF-type DNA-binding-PF00447,SUPERFAMILY--SSF46785 compare
mRNA_7822 16190 +1.0 1.1 Hypothetical Protein compare
mRNA_5603 13971 +1.0 4.3 K09523 DNAJC3 DnaJ homolog subfamily C member 3 compare
mRNA_4899 13267 +1.0 1.4 KOG4705 Uncharacterized conserved protein compare
mRNA_8001 16369 +1.0 4.3 K01092 E3.1.3.25, IMPA, suhB myo-inositol-1(or 4)-monophosphatase compare
mRNA_1707 10075 +0.9 6.7 BLAST related to SNF1-related protein kinase KIN10 [Melanop... compare
mRNA_3770 12138 +0.9 2.5 K12606 RCD1, CNOT9, CAF40 CCR4-NOT transcription complex subunit 9 compare
mRNA_6485 14853 +0.9 1.9 K00826 E2.6.1.42, ilvE branched-chain amino acid aminotransferase compare
mRNA_6428 14796 +0.9 7.3 KOG2208 Vigilin compare
mRNA_6438 14806 +0.9 1.8 K15456 KTI12 protein KTI12 compare
mRNA_4985 13353 +0.9 1.2 BLAST vps9 domain protein [Zymoseptoria brevis] compare
mRNA_7297 15665 +0.9 3.1 Hypothetical Protein compare
mRNA_775 9143 +0.9 2.7 Hypothetical Protein compare
mRNA_158 8526 +0.9 1.9 BLAST expressed protein [Cryptococcus neoformans var. neofo... compare
mRNA_5644 14012 +0.9 2.8 Hypothetical Protein compare
mRNA_7369 15737 +0.9 1.4 KOG1203 Predicted dehydrogenase compare
mRNA_7396 15764 +0.9 1.4 K18584 ACTR3, ARP3 actin-related protein 3 compare
mRNA_8355 16723 +0.9 2.4 KOG2111 Uncharacterized conserved protein, contains WD40 repeats compare
mRNA_5133 13501 +0.9 4.1 Interpro Proteasome assembly chaperone 4 compare
mRNA_387 8755 +0.9 1.6 Hypothetical Protein compare
mRNA_4459 12827 +0.9 5.6 HMMPfam-PH domain-PF00169,ProSiteProfiles-PH domain profile.-PS50003,SMART-Pleckstrin homology domain.-SM00233,SUPERFAMILY--SSF50729 compare
mRNA_402 8770 +0.9 9.6 BLAST N-terminal acetyltransferase catalytic subunit Nat1 [... compare
mRNA_5518 13886 +0.9 2.0 SUPERFAMILY--SSF49599 compare
mRNA_722 9090 +0.9 2.2 HMMPfam-Thiopurine S-methyltransferase (TPMT)-PF05724,ProSiteProfiles-Thiopurine or thiol or thiocyanate S-methyltransferase (TPMT) family profile.-PS51585,SUPERFAMILY--SSF53335 compare
mRNA_1055 9423 +0.9 1.2 KOG0590 Checkpoint kinase and related serine/threonine protein kinases compare
mRNA_703 9071 +0.9 4.5 K20223 IPO7, RANBP7 importin-7 compare
mRNA_2527 10895 +0.9 3.3 BLAST protein DGCR14-like [Mucor ambiguus] compare
mRNA_1746 10114 +0.9 1.7 KOG2897 DNA-binding protein YL1 and related proteins compare
mRNA_1260 9628 +0.9 8.6 Hypothetical Protein compare
mRNA_6215 14583 +0.9 2.6 Hypothetical Protein compare
mRNA_7499 15867 +0.9 3.4 Hypothetical Protein compare
mRNA_2233 10601 +0.9 1.3 Hypothetical Protein compare
mRNA_6074 14442 +0.9 1.7 Hypothetical Protein compare
mRNA_6560 14928 +0.9 1.2 Hypothetical Protein compare
mRNA_4806 13174 +0.9 3.0 Hypothetical Protein compare
mRNA_7921 16289 +0.9 0.9 KOG3022 Predicted ATPase, nucleotide-binding compare
mRNA_2082 10450 +0.9 2.8 Hypothetical Protein compare
mRNA_7078 15446 +0.9 0.8 Hypothetical Protein compare
mRNA_657 9025 +0.9 1.8 Hypothetical Protein compare
mRNA_2352 10720 +0.9 1.5 K02903 RP-L28e, RPL28 large subunit ribosomal protein L28e compare
mRNA_7100 15468 +0.9 2.8 BLAST putative protein [Melanopsichium pennsylvanicum 4] compare
mRNA_1384 9752 +0.9 2.4 KOG4472 Glycolipid 2-alpha-mannosyltransferase (alpha-1,2-mannosyltransferase) compare
mRNA_6843 15211 +0.9 4.1 BLAST protein of unknown function [Taphrina deformans PYCC ... compare
mRNA_5794 14162 +0.9 4.0 K01637 E4.1.3.1, aceA isocitrate lyase compare
mRNA_1741 10109 +0.8 1.7 HMMPfam-Calcipressin-PF04847 compare
mRNA_6368 14736 +0.8 1.3 KOG2049 Translational repressor MPT5/PUF4 and related RNA-binding proteins (Puf superfamily) compare
mRNA_6685 15053 +0.8 1.3 Hypothetical Protein compare
mRNA_6242 14610 +0.8 3.6 K00826 E2.6.1.42, ilvE branched-chain amino acid aminotransferase compare
mRNA_6374 14742 +0.8 5.9 K20464 OSBPL5_8, ORP5_8 oxysterol-binding protein-related protein 5/8 compare
mRNA_1834 10202 +0.8 2.4 Hypothetical Protein compare
mRNA_3623 11991 +0.8 2.7 HMMPfam-RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)-PF00076,ProSiteProfiles-Eukaryotic RNA Recognition Motif (RRM) profile.-PS50102,SMART-RNA recognition motif-SM00360,SUPERFAMILY--SSF54928 compare
mRNA_5216 13584 +0.8 1.1 K04648 DCTN1 dynactin 1 compare
mRNA_8124 16492 +0.8 2.0 HMMPfam-FHA domain-PF00498,ProSiteProfiles-Forkhead-associated (FHA) domain profile.-PS50006,SMART-Forkhead associated domain-SM00240,SUPERFAMILY--SSF49879 compare
mRNA_4218 12586 +0.8 4.5 K17491 SMEK, PPP4R3 protein phosphatase 4 regulatory subunit 3 compare
mRNA_4875 13243 +0.8 1.7 ProSitePatterns-Tyrosine protein kinases specific active-site signature.-PS00109,SUPERFAMILY--SSF56112 compare
mRNA_1046 9414 +0.8 4.5 K11346 ING4 inhibitor of growth protein 4 compare
mRNA_7674 16042 +0.8 0.7 SUPERFAMILY--SSF48371 compare
mRNA_3341 11709 +0.8 2.3 K20724 TMEM33 transmembrane protein 33 compare
mRNA_2172 10540 +0.8 2.2 K00968 PCYT1 choline-phosphate cytidylyltransferase compare
mRNA_7891 16259 +0.8 2.1 Hypothetical Protein compare
mRNA_3745 12113 +0.8 0.6 BLAST protein of unknown function [Taphrina deformans PYCC ... compare
mRNA_1614 9982 +0.8 3.9 KOG2819 Uncharacterized conserved protein compare
mRNA_8334 16702 +0.8 5.4 BLAST mtf1_muccl ame full transcription factor mtf1 [Licht... compare
mRNA_7919 16287 +0.8 2.2 K01489 cdd, CDA cytidine deaminase compare
mRNA_3834 12202 +0.8 2.0 Hypothetical Protein compare
mRNA_162 8530 +0.8 1.9 BLAST NADH dehydrogenase [ubiquinone] 1 alpha subcomplex su... compare
mRNA_7225 15593 +0.8 1.4 Hypothetical Protein compare
mRNA_4391 12759 +0.8 1.8 Hypothetical Protein compare
mRNA_6370 14738 +0.8 2.2 K15692 RNF13, RZF E3 ubiquitin-protein ligase RNF13 compare
mRNA_7689 16057 +0.8 2.5 BLAST triacylglycerol lipase [Pochonia chlamydosporia 170] compare
mRNA_2828 11196 +0.8 1.3 Hypothetical Protein compare
mRNA_1253 9621 +0.8 1.9 K04554 UBE2J2, NCUBE2, UBC6 ubiquitin-conjugating enzyme E2 J2 compare
mRNA_4836 13204 +0.8 1.3 BLAST NEK protein kinase [Microbotryum lychnidis-dioicae p1... compare
mRNA_4801 13169 +0.8 1.1 HMMPfam-Uncharacterized alpha/beta hydrolase domain (DUF2235)-PF09994 compare
mRNA_3135 11503 +0.8 2.4 HMMPfam-Alpha/beta hydrolase family-PF12695,SUPERFAMILY--SSF53474 compare
mRNA_6882 15250 +0.8 1.8 Hypothetical Protein compare
mRNA_5230 13598 +0.8 5.5 K17906 ATG2 autophagy-related protein 2 compare
mRNA_2005 10373 +0.8 1.8 K08286 E2.7.11.- protein-serine/threonine kinase compare
mRNA_1866 10234 +0.8 1.7 KOG4455 Uncharacterized conserved protein compare
mRNA_2671 11039 +0.8 2.9 K00111 glpA, glpD glycerol-3-phosphate dehydrogenase compare
mRNA_2528 10896 +0.8 1.6 HMMPfam-Fungal chitosanase of glycosyl hydrolase group 75-PF07335 compare
mRNA_5215 13583 +0.8 1.1 KOG1940 Zn-finger protein compare
mRNA_24 8392 +0.8 1.9 Hypothetical Protein compare
mRNA_5608 13976 +0.8 1.6 K03018 RPC1, POLR3A DNA-directed RNA polymerase III subunit RPC1 compare
mRNA_6205 14573 +0.8 2.5 HMMPfam-Peptidase M50B-like-PF13398 compare
mRNA_5217 13585 +0.8 2.2 Hypothetical Protein compare
mRNA_7482 15850 +0.8 1.8 Hypothetical Protein compare
mRNA_7723 16091 +0.8 1.3 HMMPfam-Domain of unknown function (DUF427)-PF04248 compare
mRNA_397 8765 +0.8 2.6 SUPERFAMILY--SSF52047 compare
mRNA_3085 11453 +0.8 3.8 K06269 PPP1C serine/threonine-protein phosphatase PP1 catalytic subunit compare
mRNA_121 8489 +0.8 1.0 BLAST Pc20g03530 [Penicillium rubens Wisconsin 54-1255] compare
mRNA_8148 16516 +0.8 1.5 HMMPfam-START domain-PF01852,ProSiteProfiles-START domain profile.-PS50848,SUPERFAMILY--SSF55961 compare
mRNA_8255 16623 +0.8 1.4 K01792 E5.1.3.15 glucose-6-phosphate 1-epimerase compare
mRNA_2126 10494 +0.8 1.8 Hypothetical Protein compare
mRNA_7239 15607 +0.8 1.9 Hypothetical Protein compare
mRNA_4506 12874 +0.8 3.1 KOG4754 Predicted phosphoglycerate mutase compare
mRNA_2452 10820 +0.7 3.0 HMMPfam-Ribonuclease H2 non-catalytic subunit (Ylr154p-like)-PF08615 compare


Specific Phenotypes

None in this experiment

For Rhodosporidium toruloides IFO0880 in facs on lipid accumulation experiments

For facs on lipid accumulation CN120 across organisms