Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9998 and 9999 are separated by 357 nucleotides9999 and 10000 are separated by 1563 nucleotides10000 and 10001 are separated by 740 nucleotides10001 and 10002 are separated by 179 nucleotides mRNA_1630: 9998 - SUPERFAMILY--SSF81383, at 756,759 to 757,602 9998 mRNA_1631: 9999 - BLAST Tyrosine-protein phosphatase non-receptor type 23 [St..., at 757,960 to 758,498 9999 mRNA_1632: 10000 - K00036 G6PD, zwf glucose-6-phosphate 1-dehydrogenase, at 760,062 to 761,705 10000 mRNA_1633: 10001 - HMMPfam-Major Facilitator Superfamily-PF07690,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 762,446 to 764,135 10001 mRNA_1634: 10002 - HMMPfam-alpha/beta hydrolase fold-PF07859,ProSitePatterns-Lipolytic enzymes G-D-X-G family, putative serine active site.-PS01174,SUPERFAMILY--SSF53474, at 764,315 to 766,210 10002
Group Condition mRNA_1630 mRNA_1631 mRNA_1632 mRNA_1633 mRNA_1634
9998 9999 10000 10001 10002
growth on oleic acid YNB Oleic Acid +0.3 -0.4 N.D. -0.8 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.6 N.D. -0.5 -0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.3 N.D. -0.4 -0.2
auxotrophy YNB Glucose 75mM Arginine +0.3 -0.3 N.D. -0.7 -0.0
growth on ricinoleic acid YNB Glucose +0.1 -0.4 N.D. -0.6 +0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.8 -0.3 N.D. -0.1 +0.5
growth on ypd YPD +0.5 +0.2 N.D. -0.7 -0.4
auxotrophy Dropout Complete -0.0 +0.1 N.D. -0.4 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.1 N.D. -0.3 -0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.2 N.D. -0.6 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.0 N.D. +0.0 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.2 N.D. +0.0 -0.5
growth on oleic acid YNB Glucose +0.7 +0.1 N.D. -0.7 -0.0
auxotrophy YNB Glucose +0.4 -0.1 N.D. -0.2 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 -0.4 N.D. +0.6 +0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 +0.1 N.D. +1.5 -1.6
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 N.D. +0.5 +0.0
ypd buoyancy 40 hrs Low Buoyancy +1.0 +0.2 N.D. +0.2 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.7 +0.3 N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 +0.6 N.D. +0.7 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.8 +0.5 N.D. +0.5 -0.1
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