Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10002 and 10003 are separated by 1003 nucleotides10003 and 10004 are separated by 263 nucleotides10004 and 10005 are separated by 182 nucleotides10005 and 10006 are separated by 816 nucleotides mRNA_1634: 10002 - HMMPfam-alpha/beta hydrolase fold-PF07859,ProSitePatterns-Lipolytic enzymes G-D-X-G family, putative serine active site.-PS01174,SUPERFAMILY--SSF53474, at 764,315 to 766,210 10002 mRNA_1635: 10003 - K18748 SSD1 protein SSD1, at 767,214 to 771,755 10003 mRNA_1636: 10004 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 772,019 to 773,712 10004 mRNA_1637: 10005 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 773,895 to 775,574 10005 mRNA_1638: 10006 - K11807 WDTC1 WD and tetratricopeptide repeats protein 1, at 776,391 to 779,009 10006
Group Condition mRNA_1634 mRNA_1635 mRNA_1636 mRNA_1637 mRNA_1638
10002 10003 10004 10005 10006
ypd buoyancy 40 hrs No Buoyancy Separation -1.6 -0.1 +0.4 +0.2 -0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.3 -0.4 -0.4 -0.1
growth on oleic acid YNB Glucose -0.0 -0.4 -0.2 -0.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 -1.3 +0.1 -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.5 -0.2 +0.0 +0.1
growth on ypd YPD -0.4 -0.0 +0.1 -0.1 -0.3
auxotrophy Dropout Complete +0.1 -0.7 -0.3 +0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.4 -0.3 -0.3 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.4 -0.7 -0.1 +0.3 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.7 -0.0 +0.1 +0.3
auxotrophy YNB Glucose +0.1 -0.5 -0.2 +0.2 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.6 +0.0 +0.4 -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.5 -0.2 -0.1 -0.1 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 -0.0 -0.3 +0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.4 +0.0 +0.1 +0.1 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.5 +0.1 +0.3 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.5 +0.1 +0.3 +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.3 +0.1 +0.2 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 +0.0 +0.1 +0.2
growth on ricinoleic acid YNB Glucose +0.1 -0.4 -0.1 +0.5 +0.4
facs on lipid accumulation Sorted High BODIPY Gate +0.5 +0.2 +0.1 -0.2 +0.1
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