Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10003 and 10004 are separated by 263 nucleotides10004 and 10005 are separated by 182 nucleotides10005 and 10006 are separated by 816 nucleotides10006 and 10007 are separated by 495 nucleotides mRNA_1635: 10003 - K18748 SSD1 protein SSD1, at 767,214 to 771,755 10003 mRNA_1636: 10004 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 772,019 to 773,712 10004 mRNA_1637: 10005 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 773,895 to 775,574 10005 mRNA_1638: 10006 - K11807 WDTC1 WD and tetratricopeptide repeats protein 1, at 776,391 to 779,009 10006 mRNA_1639: 10007 - K00164 OGDH, sucA 2-oxoglutarate dehydrogenase E1 component, at 779,505 to 783,057 10007
Group Condition mRNA_1635 mRNA_1636 mRNA_1637 mRNA_1638 mRNA_1639
10003 10004 10005 10006 10007
growth on oleic acid YNB Oleic Acid -0.3 -0.4 -0.4 -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 +0.1 -0.2 +0.3 N.D.
growth on oleic acid YNB Glucose -0.4 -0.2 -0.2 -0.1 N.D.
auxotrophy Dropout Complete -0.7 -0.3 +0.1 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -0.1 -0.1 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.4 -0.3 -0.3 +0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.5 -0.2 +0.0 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.7 -0.0 +0.1 +0.3 N.D.
auxotrophy YNB Glucose -0.5 -0.2 +0.2 +0.2 N.D.
growth on ypd YPD -0.0 +0.1 -0.1 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.6 +0.0 +0.4 -0.0 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 -0.1 +0.3 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 -0.3 +0.1 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.1 +0.3 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.2 +0.1 -0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.1 +0.2 +0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.4 +0.2 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 +0.1 +0.2 N.D.
growth on ricinoleic acid YNB Glucose -0.4 -0.1 +0.5 +0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.0 +0.1 +0.2 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.3 +0.2 +0.1 N.D.
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