Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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Top 21 experiments (either direction), sorted by average fitness

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500 nt 10005 and 10006 are separated by 816 nucleotides10006 and 10007 are separated by 495 nucleotides10007 and 10008 are separated by 716 nucleotides10008 and 10009 are separated by 473 nucleotides mRNA_1637: 10005 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 773,895 to 775,574 10005 mRNA_1638: 10006 - K11807 WDTC1 WD and tetratricopeptide repeats protein 1, at 776,391 to 779,009 10006 mRNA_1639: 10007 - K00164 OGDH, sucA 2-oxoglutarate dehydrogenase E1 component, at 779,505 to 783,057 10007 mRNA_1640: 10008 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 783,774 to 785,106 10008 mRNA_1641: 10009 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 785,580 to 788,144 10009
Group Condition mRNA_1637 mRNA_1638 mRNA_1639 mRNA_1640 mRNA_1641
10005 10006 10007 10008 10009
growth on ypd YPD -0.1 -0.3 N.D. +0.0 -0.3
auxotrophy Dropout Complete +0.1 +0.1 N.D. +0.0 -0.8
growth on oleic acid YNB Oleic Acid -0.4 -0.1 N.D. +0.2 -0.3
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.2 N.D. -0.2 -0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.1 N.D. -0.3 +0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.1 -0.2 N.D. -0.4 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.3 +0.3 N.D. +0.3 -0.6
growth on oleic acid YNB Glucose -0.2 -0.1 N.D. +0.2 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 N.D. +0.2 -0.7
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.2 N.D. -0.1 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.3 N.D. -0.2 -0.2
facs on lipid accumulation Sorted Low BODIPY Gate -0.2 +0.3 N.D. -0.2 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.0 N.D. +0.3 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 -0.0 N.D. +0.3 -0.6
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.1 N.D. +0.2 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.3 N.D. +0.1 -0.3
auxotrophy YNB Glucose +0.2 +0.2 N.D. +0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 N.D. +0.4 -0.4
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.2 N.D. +0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Glucose +0.5 +0.4 N.D. +0.2 -0.2
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