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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10006 and 10007 are separated by 495 nucleotides
10007 and 10008 are separated by 716 nucleotides
10008 and 10009 are separated by 473 nucleotides
10009 and 10010 are separated by 607 nucleotides
mRNA_1638: 10006 - K11807 WDTC1 WD and tetratricopeptide repeats protein 1, at 776,391 to 779,009
10006
mRNA_1639: 10007 - K00164 OGDH, sucA 2-oxoglutarate dehydrogenase E1 component, at 779,505 to 783,057
10007
mRNA_1640: 10008 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 783,774 to 785,106
10008
mRNA_1641: 10009 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 785,580 to 788,144
10009
mRNA_1642: 10010 - K03937 NDUFS4 NADH dehydrogenase (ubiquinone) Fe-S protein 4, at 788,752 to 789,941
10010
Group
Condition
mRNA
_1638
mRNA
_1639
mRNA
_1640
mRNA
_1641
mRNA
_1642
10006
10007
10008
10009
10010
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
N.D.
-0.2
-0.2
-1.3
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
N.D.
+0.2
-0.7
-1.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
N.D.
-0.2
-0.2
-0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.3
N.D.
+0.1
-0.3
-0.9
growth on oleic acid
YNB Oleic Acid
-0.1
N.D.
+0.2
-0.3
-0.5
auxotrophy
Dropout Complete
+0.1
N.D.
+0.0
-0.8
-0.0
growth on ypd
YPD
-0.3
N.D.
+0.0
-0.3
-0.2
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.0
N.D.
+0.3
-0.6
-0.3
ypd buoyancy
40 hrs Low Buoyancy
+0.2
N.D.
-0.1
-0.2
-0.3
growth on oleic acid
YNB Glucose
-0.1
N.D.
+0.2
-0.2
-0.2
facs on lipid accumulation
Sorted High BODIPY Gate
+0.1
N.D.
-0.3
+0.0
+0.0
auxotrophy
YNB Glucose
+0.2
N.D.
+0.2
-0.1
-0.3
facs on lipid accumulation
Sorted No BODIPY Gate
-0.2
N.D.
-0.4
+0.3
+0.3
auxotrophy
YNB Glucose 75mM Arginine
+0.3
N.D.
+0.3
-0.6
+0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.1
N.D.
+0.4
-0.4
+0.1
auxotrophy
YNB Glucose 75mM Methionine
+0.1
N.D.
+0.2
-0.1
+0.1
growth on ricinoleic acid
YNB Glucose
+0.4
N.D.
+0.2
-0.2
-0.2
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.3
N.D.
-0.2
+0.0
+0.2
ypd buoyancy
40 hrs High Buoyancy
+0.1
N.D.
+0.1
+0.1
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.2
N.D.
+0.0
+0.1
+0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.0
N.D.
+0.3
-0.5
+1.1
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