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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10008 and 10009 are separated by 473 nucleotides
10009 and 10010 are separated by 607 nucleotides
10010 and 10011 are separated by 484 nucleotides
10011 and 10012 are separated by 172 nucleotides
mRNA_1640: 10008 - KOG1208 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 783,774 to 785,106
10008
mRNA_1641: 10009 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 785,580 to 788,144
10009
mRNA_1642: 10010 - K03937 NDUFS4 NADH dehydrogenase (ubiquinone) Fe-S protein 4, at 788,752 to 789,941
10010
mRNA_1643: 10011 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 790,426 to 792,624
10011
mRNA_1644: 10012 - K00253 IVD, ivd isovaleryl-CoA dehydrogenase, at 792,797 to 794,915
10012
Group
Condition
mRNA
_1640
mRNA
_1641
mRNA
_1642
mRNA
_1643
mRNA
_1644
10008
10009
10010
10011
10012
buoyancy on lipid accumulation
40 hrs Median Buoyancy
+0.2
-0.7
-1.1
-0.3
-0.0
growth on ypd
YPD
+0.0
-0.3
-0.2
-0.4
-0.6
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.2
-0.2
-1.3
+0.4
+0.1
ypd buoyancy
40 hrs No Buoyancy Separation
-0.2
-0.2
-0.2
-0.2
-0.3
auxotrophy
Dropout Complete
+0.0
-0.8
-0.0
+0.4
-0.6
ypd buoyancy
40 hrs Low Buoyancy
-0.1
-0.2
-0.3
-0.1
-0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.3
-0.6
-0.3
-0.1
-0.0
growth on oleic acid
YNB Oleic Acid
+0.2
-0.3
-0.5
+0.4
-0.2
auxotrophy
YNB Glucose 75mM Arginine
+0.3
-0.6
+0.2
+0.2
-0.6
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.1
-0.3
-0.9
+0.7
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.2
+0.0
+0.2
-0.0
-0.1
facs on lipid accumulation
Sorted High BODIPY Gate
-0.3
+0.0
+0.0
-0.1
+0.3
growth on oleic acid
YNB Glucose
+0.2
-0.2
-0.2
+0.2
-0.0
facs on lipid accumulation
Sorted No BODIPY Gate
-0.4
+0.3
+0.3
-0.3
+0.1
auxotrophy
YNB Glucose
+0.2
-0.1
-0.3
+0.7
-0.4
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
-0.5
+1.1
-0.7
-0.2
auxotrophy
YNB Glucose 75mM Methionine
+0.2
-0.1
+0.1
+0.4
-0.5
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
+0.4
-0.4
+0.1
+0.1
-0.1
ypd buoyancy
40 hrs High Buoyancy
+0.1
+0.1
+0.2
+0.1
-0.3
ypd buoyancy
40 hrs Median Buoyancy
+0.0
+0.1
+0.3
+0.1
-0.0
growth on ricinoleic acid
YNB Glucose
+0.2
-0.2
-0.2
+0.5
+0.4
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