Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10010 and 10011 are separated by 484 nucleotides10011 and 10012 are separated by 172 nucleotides10012 and 10013 are separated by 176 nucleotides10013 and 10014 are separated by 334 nucleotides mRNA_1642: 10010 - K03937 NDUFS4 NADH dehydrogenase (ubiquinone) Fe-S protein 4, at 788,752 to 789,941 10010 mRNA_1643: 10011 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 790,426 to 792,624 10011 mRNA_1644: 10012 - K00253 IVD, ivd isovaleryl-CoA dehydrogenase, at 792,797 to 794,915 10012 mRNA_1645: 10013 - Hypothetical Protein, at 795,092 to 795,670 10013 mRNA_1646: 10014 - KOG2182 Hydrolytic enzymes of the alpha/beta hydrolase fold, at 796,005 to 798,167 10014
Group Condition mRNA_1642 mRNA_1643 mRNA_1644 mRNA_1645 mRNA_1646
10010 10011 10012 10013 10014
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.1 -0.3 -0.0 N.D. +0.2
growth on ypd YPD -0.2 -0.4 -0.6 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -1.3 +0.4 +0.1 N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.5 +0.4 -0.2 N.D. -0.5
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.2 -0.6 N.D. -0.6
ypd buoyancy 40 hrs Low Buoyancy -0.3 -0.1 -0.0 N.D. -0.2
auxotrophy Dropout Complete -0.0 +0.4 -0.6 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.2 -0.3 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.4 -0.5 N.D. -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.3 -0.3 +0.1 N.D. -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.3 -0.1 -0.0 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.1 +0.3 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.1 -0.3 N.D. -0.3
growth on oleic acid YNB Glucose -0.2 +0.2 -0.0 N.D. -0.2
auxotrophy YNB Glucose -0.3 +0.7 -0.4 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.0 -0.1 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.9 +0.7 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.1 -0.1 N.D. -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.1 -0.0 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +1.1 -0.7 -0.2 N.D. +0.3
growth on ricinoleic acid YNB Glucose -0.2 +0.5 +0.4 N.D. +0.1
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