Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10011 and 10012 are separated by 172 nucleotides10012 and 10013 are separated by 176 nucleotides10013 and 10014 are separated by 334 nucleotides10014 and 10015 are separated by 2003 nucleotides mRNA_1643: 10011 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 790,426 to 792,624 10011 mRNA_1644: 10012 - K00253 IVD, ivd isovaleryl-CoA dehydrogenase, at 792,797 to 794,915 10012 mRNA_1645: 10013 - Hypothetical Protein, at 795,092 to 795,670 10013 mRNA_1646: 10014 - KOG2182 Hydrolytic enzymes of the alpha/beta hydrolase fold, at 796,005 to 798,167 10014 mRNA_1647: 10015 - K02930 RP-L4e, RPL4 large subunit ribosomal protein L4e, at 800,171 to 801,820 10015
Group Condition mRNA_1643 mRNA_1644 mRNA_1645 mRNA_1646 mRNA_1647
10011 10012 10013 10014 10015
growth on ypd YPD -0.4 -0.6 N.D. -0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.6 N.D. -0.6 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.1 N.D. -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 -0.2 N.D. +0.3 N.D.
auxotrophy Dropout Complete +0.4 -0.6 N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.4 -0.5 N.D. -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.3 N.D. -0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.4 -0.2 N.D. -0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 N.D. -0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.3 N.D. -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 -0.3 N.D. +0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.1 N.D. -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 -0.0 N.D. -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 -0.0 N.D. +0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 N.D. -0.2 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 -0.1 N.D. -0.1 N.D.
growth on oleic acid YNB Glucose +0.2 -0.0 N.D. -0.2 N.D.
auxotrophy YNB Glucose +0.7 -0.4 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 +0.1 N.D. -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 +0.1 N.D. +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.5 +0.4 N.D. +0.1 N.D.
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