Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10013 and 10014 are separated by 334 nucleotides10014 and 10015 are separated by 2003 nucleotides10015 and 10016 are separated by 461 nucleotides10016 and 10017 are separated by 131 nucleotides mRNA_1645: 10013 - Hypothetical Protein, at 795,092 to 795,670 10013 mRNA_1646: 10014 - KOG2182 Hydrolytic enzymes of the alpha/beta hydrolase fold, at 796,005 to 798,167 10014 mRNA_1647: 10015 - K02930 RP-L4e, RPL4 large subunit ribosomal protein L4e, at 800,171 to 801,820 10015 mRNA_1648: 10016 - K11436 PRMT3 type I protein arginine methyltransferase, at 802,282 to 804,374 10016 mRNA_1649: 10017 - KOG2495 NADH-dehydrogenase (ubiquinone), at 804,506 to 806,689 10017
Group Condition mRNA_1645 mRNA_1646 mRNA_1647 mRNA_1648 mRNA_1649
10013 10014 10015 10016 10017
ypd buoyancy 40 hrs High Buoyancy N.D. -0.3 N.D. N.D. -0.3
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 N.D. N.D. -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.2 N.D. N.D. -0.7
growth on ypd YPD N.D. -0.1 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.6 N.D. N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.5 N.D. N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.2 N.D. N.D. -0.0
auxotrophy Dropout Complete N.D. -0.3 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 N.D. N.D. +0.3
growth on oleic acid YNB Oleic Acid N.D. -0.5 N.D. N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.4 N.D. N.D. +0.4
auxotrophy YNB Glucose N.D. -0.1 N.D. N.D. +0.1
growth on oleic acid YNB Glucose N.D. -0.2 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.1 N.D. N.D. +0.5
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.2 N.D. N.D. +0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.0 N.D. N.D. +0.5
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 N.D. N.D. +0.5
growth on ricinoleic acid YNB Glucose N.D. +0.1 N.D. N.D. +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 N.D. N.D. +0.7
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.3 N.D. N.D. +0.5
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