Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10015 and 10016 are separated by 461 nucleotides10016 and 10017 are separated by 131 nucleotides10017 and 10018 are separated by 886 nucleotides10018 and 10019 are separated by 193 nucleotides mRNA_1647: 10015 - K02930 RP-L4e, RPL4 large subunit ribosomal protein L4e, at 800,171 to 801,820 10015 mRNA_1648: 10016 - K11436 PRMT3 type I protein arginine methyltransferase, at 802,282 to 804,374 10016 mRNA_1649: 10017 - KOG2495 NADH-dehydrogenase (ubiquinone), at 804,506 to 806,689 10017 mRNA_1650: 10018 - KOG0254 Predicted transporter (major facilitator superfamily), at 807,576 to 811,155 10018 mRNA_1651: 10019 - SUPERFAMILY--SSF69047, at 811,349 to 811,874 10019
Group Condition mRNA_1647 mRNA_1648 mRNA_1649 mRNA_1650 mRNA_1651
10015 10016 10017 10018 10019
growth on ypd YPD N.D. N.D. -0.3 -0.4 -0.0
auxotrophy Dropout Complete N.D. N.D. +0.1 -0.8 +0.0
auxotrophy YNB Glucose N.D. N.D. +0.1 -0.6 +0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.3 -0.7 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.7 +0.0 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.6 -0.7 +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.3 +0.2 -0.0
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.2 +0.1 +0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.3 -0.7 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.5 -1.0 +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.5 -0.4 +0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.4 -0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.3 -0.3 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.5 -0.2 -0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.4 -0.2 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.3 -0.2 +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.0 +0.3 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.7 -0.1 -0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.4 +0.1 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.5 -0.1 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.5 +0.4 +0.1
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