Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10022 and 10023 are separated by 1820 nucleotides10023 and 10024 are separated by 121 nucleotides10024 and 10025 are separated by 489 nucleotides10025 and 10026 are separated by 1146 nucleotides mRNA_1654: 10022 - BLAST expressed protein [Cryptococcus neoformans var. neofo..., at 816,263 to 817,600 10022 mRNA_1655: 10023 - HMMPfam-PQ loop repeat-PF04193,SMART-Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.-SM00679, at 819,421 to 820,962 10023 mRNA_1656: 10024 - HMMPfam-Universal stress protein family-PF00582,PRINTS-Universal stress protein signature-PR01438,SUPERFAMILY--SSF52402, at 821,084 to 822,700 10024 mRNA_1657: 10025 - K08286 E2.7.11.- protein-serine/threonine kinase, at 823,190 to 825,684 10025 mRNA_1658: 10026 - K02893 RP-L23Ae, RPL23A large subunit ribosomal protein L23Ae, at 826,831 to 828,048 10026
Group Condition mRNA_1654 mRNA_1655 mRNA_1656 mRNA_1657 mRNA_1658
10022 10023 10024 10025 10026
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 -0.4 N.D. -4.8 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.4 +0.1 N.D. -4.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.0 -0.0 N.D. -3.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 +0.0 N.D. -1.3 N.D.
growth on oleic acid YNB Oleic Acid -0.3 +0.1 N.D. -1.9 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.6 +0.2 N.D. -1.1 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.6 +0.3 N.D. -0.7 N.D.
auxotrophy Dropout Complete -1.0 +0.2 N.D. -0.1 N.D.
auxotrophy YNB Glucose +0.1 +0.1 N.D. -1.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.3 -0.2 N.D. -0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.2 -0.0 N.D. -0.1 N.D.
growth on ricinoleic acid YNB Glucose +1.3 +0.2 N.D. -1.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.7 +0.2 N.D. +0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 N.D. -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 -0.0 N.D. +0.1 N.D.
growth on oleic acid YNB Glucose +0.3 +0.5 N.D. -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +1.2 +0.3 N.D. -1.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 +0.0 N.D. +0.5 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -1.9 +0.3 N.D. +2.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 -0.0 N.D. +1.0 N.D.
growth on ypd YPD +1.3 +0.5 N.D. +0.2 N.D.
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