Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10023 and 10024 are separated by 121 nucleotides10024 and 10025 are separated by 489 nucleotides10025 and 10026 are separated by 1146 nucleotides10026 and 10027 are separated by 810 nucleotides mRNA_1655: 10023 - HMMPfam-PQ loop repeat-PF04193,SMART-Repeated motif present between transmembrane helices in cystinosin, yeast ERS1p, mannose-P-dolichol utilization defect 1, and other hypothetical proteins.-SM00679, at 819,421 to 820,962 10023 mRNA_1656: 10024 - HMMPfam-Universal stress protein family-PF00582,PRINTS-Universal stress protein signature-PR01438,SUPERFAMILY--SSF52402, at 821,084 to 822,700 10024 mRNA_1657: 10025 - K08286 E2.7.11.- protein-serine/threonine kinase, at 823,190 to 825,684 10025 mRNA_1658: 10026 - K02893 RP-L23Ae, RPL23A large subunit ribosomal protein L23Ae, at 826,831 to 828,048 10026 mRNA_1659: 10027 - K08741 MSH5 DNA mismatch repair protein MSH5, at 828,859 to 833,266 10027
Group Condition mRNA_1655 mRNA_1656 mRNA_1657 mRNA_1658 mRNA_1659
10023 10024 10025 10026 10027
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 N.D. -4.8 N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 N.D. -4.3 N.D. +0.2
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. -3.5 N.D. +0.1
growth on oleic acid YNB Oleic Acid +0.1 N.D. -1.9 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 N.D. -1.3 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.2 N.D. -1.7 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.7 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. -1.1 N.D. -0.0
auxotrophy YNB Glucose +0.1 N.D. -1.0 N.D. -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.3 N.D. -1.0 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine +0.3 N.D. -0.7 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy -0.0 N.D. -0.1 N.D. +0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.0 N.D. -0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. +0.1 N.D. +0.1
growth on oleic acid YNB Glucose +0.5 N.D. -0.3 N.D. +0.1
auxotrophy Dropout Complete +0.2 N.D. -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. +0.5 N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 N.D. +0.5 N.D. +0.0
growth on ypd YPD +0.5 N.D. +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 N.D. +1.0 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 N.D. +2.7 N.D. -0.2
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