Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10024 and 10025 are separated by 489 nucleotides10025 and 10026 are separated by 1146 nucleotides10026 and 10027 are separated by 810 nucleotides10027 and 10028 are separated by 167 nucleotides mRNA_1656: 10024 - HMMPfam-Universal stress protein family-PF00582,PRINTS-Universal stress protein signature-PR01438,SUPERFAMILY--SSF52402, at 821,084 to 822,700 10024 mRNA_1657: 10025 - K08286 E2.7.11.- protein-serine/threonine kinase, at 823,190 to 825,684 10025 mRNA_1658: 10026 - K02893 RP-L23Ae, RPL23A large subunit ribosomal protein L23Ae, at 826,831 to 828,048 10026 mRNA_1659: 10027 - K08741 MSH5 DNA mismatch repair protein MSH5, at 828,859 to 833,266 10027 mRNA_1660: 10028 - Hypothetical Protein, at 833,434 to 834,294 10028
Group Condition mRNA_1656 mRNA_1657 mRNA_1658 mRNA_1659 mRNA_1660
10024 10025 10026 10027 10028
ypd buoyancy 40 hrs Low Buoyancy N.D. -3.5 N.D. +0.1 -1.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -4.8 N.D. -0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -4.3 N.D. +0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.3 N.D. -0.1 -1.3
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.0 N.D. +0.1 -1.6
growth on oleic acid YNB Oleic Acid N.D. -1.9 N.D. -0.0 -0.2
growth on ricinoleic acid YNB Glucose N.D. -1.7 N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.0 N.D. -0.1 -1.3
auxotrophy YNB Glucose N.D. -1.0 N.D. -0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine N.D. -0.7 N.D. -0.2 -0.4
auxotrophy Dropout Complete N.D. -0.1 N.D. +0.1 -0.9
auxotrophy YNB Glucose 75mM Arginine N.D. -0.7 N.D. +0.0 -0.1
growth on oleic acid YNB Glucose N.D. -0.3 N.D. +0.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -1.1 N.D. -0.0 +0.6
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 N.D. +0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.5 N.D. -0.1 -0.6
growth on ypd YPD N.D. +0.2 N.D. +0.0 +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 N.D. +0.1 +0.8
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +1.0 N.D. +0.1 +0.4
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +2.7 N.D. -0.2 -1.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.5 N.D. +0.0 +1.3
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