Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10035 and 10036 are separated by 957 nucleotides10036 and 10037 are separated by 297 nucleotides10037 and 10038 are separated by 376 nucleotides10038 and 10039 are separated by 708 nucleotides mRNA_1667: 10035 - K11527 K11527 two-component system, sensor histidine kinase and response regulator, at 842,295 to 847,609 10035 mRNA_1668: 10036 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 848,567 to 849,208 10036 mRNA_1669: 10037 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 849,506 to 850,957 10037 mRNA_1670: 10038 - K15918 GLYK D-glycerate 3-kinase, at 851,334 to 852,644 10038 mRNA_1671: 10039 - K13344 PEX13 peroxin-13, at 853,353 to 854,767 10039
Group Condition mRNA_1667 mRNA_1668 mRNA_1669 mRNA_1670 mRNA_1671
10035 10036 10037 10038 10039
auxotrophy Dropout Complete -0.2 -0.6 +0.2 -0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -1.2 -0.1 +0.6 N.D.
growth on oleic acid YNB Oleic Acid -0.2 +0.2 -0.1 -0.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 +0.1 -0.5 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.3 +0.0 -0.0 N.D.
growth on oleic acid YNB Glucose +0.2 +0.0 -0.2 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.2 -0.3 +0.1 +0.0 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.2 -0.8 +0.2 +0.3 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 +0.1 -0.1 -0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 -0.0 +0.2 +0.1 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 -0.1 -0.3 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 +0.0 +0.5 -0.4 N.D.
growth on ricinoleic acid YNB Glucose +0.1 -0.2 +0.4 +0.0 N.D.
auxotrophy YNB Glucose -0.1 +0.3 +0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.3 +0.3 +0.1 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.0 +0.4 +0.1 +0.1 N.D.
growth on ypd YPD -0.1 +0.9 -0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.4 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.3 +0.3 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 +0.3 +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.2 +0.6 +0.7 -0.1 N.D.
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