Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10036 and 10037 are separated by 297 nucleotides10037 and 10038 are separated by 376 nucleotides10038 and 10039 are separated by 708 nucleotides10039 and 10040 are separated by 620 nucleotides mRNA_1668: 10036 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 848,567 to 849,208 10036 mRNA_1669: 10037 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 849,506 to 850,957 10037 mRNA_1670: 10038 - K15918 GLYK D-glycerate 3-kinase, at 851,334 to 852,644 10038 mRNA_1671: 10039 - K13344 PEX13 peroxin-13, at 853,353 to 854,767 10039 mRNA_1672: 10040 - K00382 DLD, lpd, pdhD dihydrolipoamide dehydrogenase, at 855,388 to 857,888 10040
Group Condition mRNA_1668 mRNA_1669 mRNA_1670 mRNA_1671 mRNA_1672
10036 10037 10038 10039 10040
facs on lipid accumulation Sorted Low BODIPY Gate -1.2 -0.1 +0.6 N.D. N.D.
growth on oleic acid YNB Glucose +0.0 -0.2 -0.5 N.D. N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.1 -0.5 N.D. N.D.
auxotrophy Dropout Complete -0.6 +0.2 -0.1 N.D. N.D.
growth on oleic acid YNB Oleic Acid +0.2 -0.1 -0.4 N.D. N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 +0.2 +0.3 N.D. N.D.
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.0 -0.0 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.1 +0.0 N.D. N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 -0.0 N.D. N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.3 +0.3 N.D. N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 +0.5 -0.4 N.D. N.D.
growth on ricinoleic acid YNB Glucose -0.2 +0.4 +0.0 N.D. N.D.
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.2 +0.1 N.D. N.D.
auxotrophy YNB Glucose +0.3 +0.1 -0.0 N.D. N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.4 +0.1 +0.1 N.D. N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 +0.1 +0.2 N.D. N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.3 +0.1 +0.3 N.D. N.D.
growth on ypd YPD +0.9 -0.1 -0.0 N.D. N.D.
ypd buoyancy 40 hrs High Buoyancy +0.2 +0.3 +0.4 N.D. N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.3 +0.4 N.D. N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.6 +0.7 -0.1 N.D. N.D.
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