Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10037 and 10038 are separated by 376 nucleotides10038 and 10039 are separated by 708 nucleotides10039 and 10040 are separated by 620 nucleotides10040 and 10041 are separated by 235 nucleotides mRNA_1669: 10037 - HMMPfam-Acetyltransferase (GNAT) family-PF00583,ProSiteProfiles-Gcn5-related N-acetyltransferase (GNAT) domain profile.-PS51186,SUPERFAMILY--SSF55729, at 849,506 to 850,957 10037 mRNA_1670: 10038 - K15918 GLYK D-glycerate 3-kinase, at 851,334 to 852,644 10038 mRNA_1671: 10039 - K13344 PEX13 peroxin-13, at 853,353 to 854,767 10039 mRNA_1672: 10040 - K00382 DLD, lpd, pdhD dihydrolipoamide dehydrogenase, at 855,388 to 857,888 10040 mRNA_1673: 10041 - Hypothetical Protein, at 858,124 to 858,750 10041
Group Condition mRNA_1669 mRNA_1670 mRNA_1671 mRNA_1672 mRNA_1673
10037 10038 10039 10040 10041
growth on oleic acid YNB Glucose -0.2 -0.5 N.D. N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.5 N.D. N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.3 +0.3 N.D. N.D. -0.4
growth on ypd YPD -0.1 -0.0 N.D. N.D. -0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.0 N.D. N.D. -0.1
growth on oleic acid YNB Oleic Acid -0.1 -0.4 N.D. N.D. +0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.2 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.0 N.D. N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 +0.6 N.D. N.D. -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 N.D. N.D. -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.4 N.D. N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy +0.1 +0.1 N.D. N.D. +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 +0.3 N.D. N.D. -0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 +0.1 N.D. N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine +0.0 -0.0 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 +0.4 N.D. N.D. +0.0
growth on ricinoleic acid YNB Glucose +0.4 +0.0 N.D. N.D. +0.4
ypd buoyancy 40 hrs High Buoyancy +0.3 +0.4 N.D. N.D. +0.2
auxotrophy Dropout Complete +0.2 -0.1 N.D. N.D. +0.8
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 -0.1 N.D. N.D. +0.3
auxotrophy YNB Glucose +0.1 -0.0 N.D. N.D. +0.8
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