Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10039 and 10040 are separated by 620 nucleotides10040 and 10041 are separated by 235 nucleotides10041 and 10042 are separated by 1450 nucleotides10042 and 10043 are separated by 549 nucleotides mRNA_1671: 10039 - K13344 PEX13 peroxin-13, at 853,353 to 854,767 10039 mRNA_1672: 10040 - K00382 DLD, lpd, pdhD dihydrolipoamide dehydrogenase, at 855,388 to 857,888 10040 mRNA_1673: 10041 - Hypothetical Protein, at 858,124 to 858,750 10041 mRNA_1674: 10042 - K07297 ADIPOR adiponectin receptor, at 860,201 to 862,501 10042 mRNA_1675: 10043 - K01424 E3.5.1.1, ansA, ansB L-asparaginase, at 863,051 to 864,755 10043
Group Condition mRNA_1671 mRNA_1672 mRNA_1673 mRNA_1674 mRNA_1675
10039 10040 10041 10042 10043
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.3 -0.3 +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.4 +0.1 -0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 -0.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. -0.4 +0.3 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. -0.1 -0.1 -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. -0.1 +0.0 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. +0.3 +0.3 -0.5
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. +0.2 -0.0 -0.1
growth on ricinoleic acid YNB Glucose N.D. N.D. +0.4 +0.4 -0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.0 +0.3 -0.0
growth on oleic acid YNB Glucose N.D. N.D. -0.0 +0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.2 +0.0 +0.1
growth on ypd YPD N.D. N.D. -0.1 +0.5 -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. +0.3 +0.6 -0.4
growth on oleic acid YNB Oleic Acid N.D. N.D. +0.4 +0.5 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. -0.1 +0.5 +0.2
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.1 +0.8 -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.1 +0.4 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. +0.5 +0.7 +0.0
auxotrophy YNB Glucose N.D. N.D. +0.8 +0.5 +0.1
auxotrophy Dropout Complete N.D. N.D. +0.8 +0.8 +0.2
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