Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10077 and 10078 are separated by 560 nucleotides10078 and 10079 are separated by 319 nucleotides10079 and 10080 are separated by 494 nucleotides10080 and 10081 are separated by 331 nucleotides mRNA_1709: 10077 - K16302 CNNM metal transporter CNNM, at 5,028 to 7,438 10077 mRNA_1710: 10078 - Hypothetical Protein, at 7,999 to 8,750 10078 mRNA_1711: 10079 - K01887 RARS, argS arginyl-tRNA synthetase, at 9,070 to 11,520 10079 mRNA_1712: 10080 - KOG2131 Uncharacterized conserved protein, contains JmjC domain, at 12,015 to 13,653 10080 mRNA_1713: 10081 - KOG0022 Alcohol dehydrogenase, class III, at 13,985 to 15,656 10081
Group Condition mRNA_1709 mRNA_1710 mRNA_1711 mRNA_1712 mRNA_1713
10077 10078 10079 10080 10081
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 -0.9 N.D. -0.0 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.5 N.D. -0.2 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 -0.4 N.D. +0.0 +0.0
growth on ypd YPD +0.5 -0.6 N.D. -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.0 N.D. -0.1 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 N.D. -0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.3 N.D. -0.1 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 -0.4 N.D. +0.4 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.3 -0.1 N.D. -0.1 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 -0.2 N.D. +0.2 +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 +0.1 N.D. +0.0 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.3 N.D. +0.2 +0.1
growth on oleic acid YNB Oleic Acid +0.1 +0.5 N.D. +0.0 +0.1
growth on oleic acid YNB Glucose +0.0 +0.5 N.D. +0.0 +0.2
auxotrophy Dropout Complete -0.1 +0.6 N.D. -0.0 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.0 +0.7 N.D. -0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine -0.1 +1.2 N.D. -0.5 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 +0.4 N.D. +0.1 +0.1
growth on ricinoleic acid YNB Glucose -0.0 +0.6 N.D. +0.4 -0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.4 N.D. +0.0 +0.2
auxotrophy YNB Glucose +0.0 +1.0 N.D. +0.0 +0.0
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