Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10079 and 10080 are separated by 494 nucleotides10080 and 10081 are separated by 331 nucleotides10081 and 10082 are separated by 326 nucleotides10082 and 10083 are separated by 545 nucleotides mRNA_1711: 10079 - K01887 RARS, argS arginyl-tRNA synthetase, at 9,070 to 11,520 10079 mRNA_1712: 10080 - KOG2131 Uncharacterized conserved protein, contains JmjC domain, at 12,015 to 13,653 10080 mRNA_1713: 10081 - KOG0022 Alcohol dehydrogenase, class III, at 13,985 to 15,656 10081 mRNA_1714: 10082 - K01372 BLMH, pepC bleomycin hydrolase, at 15,983 to 18,222 10082 mRNA_1715: 10083 - K18328 DBR1 lariat debranching enzyme, at 18,768 to 21,096 10083
Group Condition mRNA_1711 mRNA_1712 mRNA_1713 mRNA_1714 mRNA_1715
10079 10080 10081 10082 10083
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 -0.2 -0.3 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.1 -0.3 N.D.
growth on ypd YPD N.D. -0.1 -0.2 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.0 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.0 -0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.5 +0.3 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.1 +0.1 -0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.1 +0.0 +0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.1 -0.0 +0.3 N.D.
growth on oleic acid YNB Oleic Acid N.D. +0.0 +0.1 +0.1 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 +0.3 +0.2 N.D.
auxotrophy YNB Glucose N.D. +0.0 +0.0 +0.2 N.D.
growth on oleic acid YNB Glucose N.D. +0.0 +0.2 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.2 +0.1 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.0 +0.2 +0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 +0.1 +0.3 N.D.
auxotrophy Dropout Complete N.D. -0.0 +0.2 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.2 +0.3 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.4 +0.1 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.0 +0.2 +0.4 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.4 -0.0 +0.3 N.D.
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