Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10083 and 10084 are separated by 306 nucleotides10084 and 10085 are separated by 378 nucleotides10085 and 10086 are separated by 306 nucleotides10086 and 10087 are separated by 785 nucleotides mRNA_1715: 10083 - K18328 DBR1 lariat debranching enzyme, at 18,768 to 21,096 10083 mRNA_1716: 10084 - K14799 TSR1 pre-rRNA-processing protein TSR1, at 21,403 to 24,344 10084 mRNA_1717: 10085 - HMMPfam-Per1-like-PF04080, at 24,723 to 26,291 10085 mRNA_1718: 10086 - HMMPfam-Cytochrome C oxidase copper chaperone (COX17)-PF05051,SUPERFAMILY--SSF47072, at 26,598 to 27,052 10086 mRNA_1719: 10087 - Hypothetical Protein, at 27,838 to 28,571 10087
Group Condition mRNA_1715 mRNA_1716 mRNA_1717 mRNA_1718 mRNA_1719
10083 10084 10085 10086 10087
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -2.0 N.D. +0.9
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -1.5 N.D. +0.7
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.2 N.D. -0.5
auxotrophy YNB Glucose N.D. N.D. -1.1 N.D. +0.5
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. -0.8 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. -0.1 N.D. -0.4
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. -0.1 N.D. -0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -1.3 N.D. +0.9
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.0 N.D. -0.4
growth on ypd YPD N.D. N.D. -0.3 N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.8 N.D. +0.7
growth on oleic acid YNB Glucose N.D. N.D. -0.9 N.D. +0.8
auxotrophy Dropout Complete N.D. N.D. -0.5 N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.1 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.4 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. +0.1 N.D. +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.2 N.D. -0.0
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. -0.1 N.D. +0.4
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. +0.4 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.4 N.D. +0.4
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