Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10088 and 10089 are separated by 367 nucleotides10089 and 10090 are separated by 540 nucleotides10090 and 10091 are separated by 1264 nucleotides10091 and 10092 are separated by 619 nucleotides mRNA_1720: 10088 - HMMPfam-Zinc finger, C2H2 type-PF00096,ProSitePatterns-Zinc finger C2H2 type domain signature.-PS00028,ProSiteProfiles-Zinc finger C2H2 type domain profile.-PS50157,SUPERFAMILY--SSF57667, at 29,211 to 32,060 10088 mRNA_1721: 10089 - K11483 HOS2 histone deacetylase HOS2, at 32,428 to 34,334 10089 mRNA_1722: 10090 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 34,875 to 37,390 10090 mRNA_1723: 10091 - KOG4157 beta-1,6-N-acetylglucosaminyltransferase, contains WSC domain, at 38,655 to 42,471 10091 mRNA_1724: 10092 - K08827 PRPF4B serine/threonine-protein kinase PRP4, at 43,091 to 44,788 10092
Group Condition mRNA_1720 mRNA_1721 mRNA_1722 mRNA_1723 mRNA_1724
10088 10089 10090 10091 10092
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 -2.1 +0.2 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -1.2 -0.0 +0.1 N.D.
auxotrophy Dropout Complete -0.1 -1.1 -0.2 -0.0 N.D.
growth on oleic acid YNB Glucose -0.1 -0.7 -0.2 -0.2 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 -1.0 +0.0 +0.1 N.D.
growth on ricinoleic acid YNB Glucose -0.5 -0.5 -0.1 +0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 -0.7 -0.1 +0.3 N.D.
growth on oleic acid YNB Oleic Acid -0.3 -0.2 -0.0 -0.1 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.2 -0.7 -0.1 +0.0 N.D.
growth on ypd YPD -0.1 -0.3 +0.0 -0.1 N.D.
auxotrophy YNB Glucose -0.0 -0.4 -0.1 +0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 +0.1 -0.1 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 -0.1 +0.0 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.1 -0.3 -0.0 +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.1 -0.2 +0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.3 +0.3 +0.1 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid -0.2 -0.3 +0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 +0.6 +0.1 +0.2 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 +0.4 +0.1 +0.3 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.5 +0.6 +0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 +0.6 +0.2 +0.3 N.D.
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