Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10089 and 10090 are separated by 540 nucleotides10090 and 10091 are separated by 1264 nucleotides10091 and 10092 are separated by 619 nucleotides10092 and 10093 are separated by 1908 nucleotides mRNA_1721: 10089 - K11483 HOS2 histone deacetylase HOS2, at 32,428 to 34,334 10089 mRNA_1722: 10090 - KOG0065 Pleiotropic drug resistance proteins (PDR1-15), ABC superfamily, at 34,875 to 37,390 10090 mRNA_1723: 10091 - KOG4157 beta-1,6-N-acetylglucosaminyltransferase, contains WSC domain, at 38,655 to 42,471 10091 mRNA_1724: 10092 - K08827 PRPF4B serine/threonine-protein kinase PRP4, at 43,091 to 44,788 10092 mRNA_1725: 10093 - KOG2343 Glucose-repressible protein and related proteins, at 46,697 to 49,542 10093
Group Condition mRNA_1721 mRNA_1722 mRNA_1723 mRNA_1724 mRNA_1725
10089 10090 10091 10092 10093
facs on lipid accumulation Sorted Low BODIPY Gate -2.1 +0.2 -0.0 N.D. +0.2
facs on lipid accumulation Sorted High BODIPY Gate -1.2 -0.0 +0.1 N.D. -0.4
auxotrophy Dropout Complete -1.1 -0.2 -0.0 N.D. +0.3
facs on lipid accumulation Sorted No BODIPY Gate -1.0 +0.0 +0.1 N.D. -0.1
growth on oleic acid YNB Glucose -0.7 -0.2 -0.2 N.D. +0.4
auxotrophy YNB Glucose 75mM Methionine -0.7 -0.1 +0.0 N.D. +0.4
ypd buoyancy 40 hrs Median Buoyancy -0.3 -0.0 +0.0 N.D. -0.0
growth on ricinoleic acid YNB Glucose -0.5 -0.1 +0.1 N.D. +0.1
growth on ypd YPD -0.3 +0.0 -0.1 N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 +0.1 -0.1 N.D. -0.5
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.1 -0.1 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.2 -0.1 +0.0 N.D. +0.2
auxotrophy YNB Glucose -0.4 -0.1 +0.1 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.7 -0.1 +0.3 N.D. +0.7
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.2 +0.1 N.D. +0.3
growth on oleic acid YNB Oleic Acid -0.2 -0.0 -0.1 N.D. +1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.6 +0.1 +0.2 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 +0.2 +0.3 N.D. -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.1 +0.3 N.D. +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.6 +0.1 +0.4 N.D. +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.3 +0.1 +0.4 N.D. +1.3
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