Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10091 and 10092 are separated by 619 nucleotides10092 and 10093 are separated by 1908 nucleotides10093 and 10094 are separated by 509 nucleotides10094 and 10095 are separated by 1133 nucleotides mRNA_1723: 10091 - KOG4157 beta-1,6-N-acetylglucosaminyltransferase, contains WSC domain, at 38,655 to 42,471 10091 mRNA_1724: 10092 - K08827 PRPF4B serine/threonine-protein kinase PRP4, at 43,091 to 44,788 10092 mRNA_1725: 10093 - KOG2343 Glucose-repressible protein and related proteins, at 46,697 to 49,542 10093 mRNA_1726: 10094 - BLAST Protein priA [Grifola frondosa], at 50,052 to 51,969 10094 mRNA_1727: 10095 - K03843 ALG2 alpha-1,3/alpha-1,6-mannosyltransferase, at 53,103 to 55,256 10095
Group Condition mRNA_1723 mRNA_1724 mRNA_1725 mRNA_1726 mRNA_1727
10091 10092 10093 10094 10095
facs on lipid accumulation Sorted High BODIPY Gate +0.1 N.D. -0.4 -0.5 N.D.
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.1 -0.8 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 N.D. -0.5 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. +0.2 -0.8 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.0 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.1 N.D. +0.0 -0.3 N.D.
growth on ypd YPD -0.1 N.D. +0.2 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.0 +0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.1 N.D. +0.3 +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. +0.4 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. +0.3 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.2 -0.1 N.D.
auxotrophy YNB Glucose +0.1 N.D. +0.4 +0.1 N.D.
growth on ricinoleic acid YNB Glucose +0.1 N.D. +0.1 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.3 N.D. -0.0 +0.5 N.D.
auxotrophy Dropout Complete -0.0 N.D. +0.3 +0.5 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.0 N.D. +0.2 +0.6 N.D.
growth on oleic acid YNB Glucose -0.2 N.D. +0.4 +0.6 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. +0.7 +0.9 N.D.
growth on oleic acid YNB Oleic Acid -0.1 N.D. +1.1 +0.9 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.4 N.D. +1.3 +1.4 N.D.
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