Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10094 and 10095 are separated by 1133 nucleotides10095 and 10096 are separated by 439 nucleotides10096 and 10097 are separated by 472 nucleotides10097 and 10098 are separated by 331 nucleotides mRNA_1726: 10094 - BLAST Protein priA [Grifola frondosa], at 50,052 to 51,969 10094 mRNA_1727: 10095 - K03843 ALG2 alpha-1,3/alpha-1,6-mannosyltransferase, at 53,103 to 55,256 10095 mRNA_1728: 10096 - HMMPfam-Fungal specific transcription factor domain-PF04082,SMART-Fungal specific transcription factor domain-SM00906, at 55,696 to 58,007 10096 mRNA_1729: 10097 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 58,480 to 59,986 10097 mRNA_1730: 10098 - KOG4708 Mitochondrial ribosomal protein MRP17, at 60,318 to 61,237 10098
Group Condition mRNA_1726 mRNA_1727 mRNA_1728 mRNA_1729 mRNA_1730
10094 10095 10096 10097 10098
facs on lipid accumulation Sorted Low BODIPY Gate -0.8 N.D. -0.5 -0.3 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.5 N.D. -0.0 -0.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.8 N.D. -0.3 -0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 N.D. -0.5 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.4 -0.2 N.D.
growth on ypd YPD +0.0 N.D. -0.2 -0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. -0.2 +0.0 N.D.
auxotrophy YNB Glucose +0.1 N.D. -0.1 -0.4 N.D.
ypd buoyancy 40 hrs Median Buoyancy -0.4 N.D. +0.0 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. -0.2 -0.4 N.D.
auxotrophy Dropout Complete +0.5 N.D. -0.2 -0.4 N.D.
growth on oleic acid YNB Glucose +0.6 N.D. -0.3 -0.4 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.6 N.D. -0.2 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. +0.1 -0.2 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. -0.0 +0.3 N.D.
growth on oleic acid YNB Oleic Acid +0.9 N.D. -0.3 -0.4 N.D.
growth on ricinoleic acid YNB Glucose +0.4 N.D. +0.3 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 N.D. +0.5 +0.3 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.9 N.D. +0.4 -0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +1.4 N.D. +0.8 +0.3 N.D.
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