Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10095 and 10096 are separated by 439 nucleotides10096 and 10097 are separated by 472 nucleotides10097 and 10098 are separated by 331 nucleotides10098 and 10099 are separated by 852 nucleotides mRNA_1727: 10095 - K03843 ALG2 alpha-1,3/alpha-1,6-mannosyltransferase, at 53,103 to 55,256 10095 mRNA_1728: 10096 - HMMPfam-Fungal specific transcription factor domain-PF04082,SMART-Fungal specific transcription factor domain-SM00906, at 55,696 to 58,007 10096 mRNA_1729: 10097 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 58,480 to 59,986 10097 mRNA_1730: 10098 - KOG4708 Mitochondrial ribosomal protein MRP17, at 60,318 to 61,237 10098 mRNA_1731: 10099 - Hypothetical Protein, at 62,090 to 63,277 10099
Group Condition mRNA_1727 mRNA_1728 mRNA_1729 mRNA_1730 mRNA_1731
10095 10096 10097 10098 10099
growth on ypd YPD N.D. -0.2 -0.4 N.D. -0.7
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.0 -0.9 N.D. -0.1
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.0 +0.3 N.D. -1.2
auxotrophy Dropout Complete N.D. -0.2 -0.4 N.D. -0.3
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.5 -0.3 N.D. -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.2 -0.4 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.2 -0.3 N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 -0.2 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy N.D. -0.2 +0.0 N.D. -0.6
growth on oleic acid YNB Glucose N.D. -0.3 -0.4 N.D. +0.0
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.0 +0.0 N.D. -0.6
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.3 -0.3 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.3 -0.4 N.D. +0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.1 -0.2 N.D. -0.3
growth on ricinoleic acid YNB Glucose N.D. +0.3 -0.3 N.D. -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.4 -0.1 N.D. -0.6
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.2 N.D. +0.4
auxotrophy YNB Glucose N.D. -0.1 -0.4 N.D. +0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.5 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.8 +0.3 N.D. +0.4
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