Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10097 and 10098 are separated by 331 nucleotides10098 and 10099 are separated by 852 nucleotides10099 and 10100 are separated by 288 nucleotides10100 and 10101 are separated by 382 nucleotides mRNA_1729: 10097 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 58,480 to 59,986 10097 mRNA_1730: 10098 - KOG4708 Mitochondrial ribosomal protein MRP17, at 60,318 to 61,237 10098 mRNA_1731: 10099 - Hypothetical Protein, at 62,090 to 63,277 10099 mRNA_1732: 10100 - Hypothetical Protein, at 63,566 to 64,770 10100 mRNA_1733: 10101 - KOG1021 Acetylglucosaminyltransferase EXT1/exostosin 1, at 65,153 to 66,958 10101
Group Condition mRNA_1729 mRNA_1730 mRNA_1731 mRNA_1732 mRNA_1733
10097 10098 10099 10100 10101
auxotrophy Dropout Complete -0.4 N.D. -0.3 -0.2 -0.5
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -1.2 -0.5 +0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 N.D. -0.3 -1.0 +0.2
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. -0.1 -0.2 -0.1
growth on oleic acid YNB Glucose -0.4 N.D. +0.0 -0.6 -0.3
growth on oleic acid YNB Oleic Acid -0.4 N.D. +0.3 -0.8 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 N.D. -0.6 -0.3 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 N.D. -0.6 -0.3 -0.1
growth on ricinoleic acid YNB Glucose -0.3 N.D. -0.3 +0.0 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.3 -0.9 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. -0.3 -0.1 +0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 N.D. -0.1 -0.5 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. -0.6 -0.2 +0.1
auxotrophy YNB Glucose -0.4 N.D. +0.4 -0.3 -0.3
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. +0.0 -0.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.4 N.D. -0.2 -0.1 +0.2
growth on ypd YPD -0.4 N.D. -0.7 +0.7 +0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.1 -0.7 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. +0.4 -0.1 -0.2
facs on lipid accumulation Sorted No BODIPY Gate -0.3 N.D. +0.1 +0.1 +0.4
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 N.D. +0.4 +0.3 -0.1
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