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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
10097 and 10098 are separated by 331 nucleotides
10098 and 10099 are separated by 852 nucleotides
10099 and 10100 are separated by 288 nucleotides
10100 and 10101 are separated by 382 nucleotides
mRNA_1729: 10097 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 58,480 to 59,986
10097
mRNA_1730: 10098 - KOG4708 Mitochondrial ribosomal protein MRP17, at 60,318 to 61,237
10098
mRNA_1731: 10099 - Hypothetical Protein, at 62,090 to 63,277
10099
mRNA_1732: 10100 - Hypothetical Protein, at 63,566 to 64,770
10100
mRNA_1733: 10101 - KOG1021 Acetylglucosaminyltransferase EXT1/exostosin 1, at 65,153 to 66,958
10101
Group
Condition
mRNA
_1729
mRNA
_1730
mRNA
_1731
mRNA
_1732
mRNA
_1733
10097
10098
10099
10100
10101
auxotrophy
Dropout Complete
-0.4
N.D.
-0.3
-0.2
-0.5
ypd buoyancy
40 hrs No Buoyancy Separation
+0.3
N.D.
-1.2
-0.5
+0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
N.D.
-0.3
-1.0
+0.2
facs on lipid accumulation
Sorted High BODIPY Gate
-0.9
N.D.
-0.1
-0.2
-0.1
growth on oleic acid
YNB Glucose
-0.4
N.D.
+0.0
-0.6
-0.3
growth on oleic acid
YNB Oleic Acid
-0.4
N.D.
+0.3
-0.8
-0.2
growth on ricinoleic acid
YNB Methylricinoleic Acid
-0.1
N.D.
-0.6
-0.3
-0.1
ypd buoyancy
40 hrs High Buoyancy
+0.0
N.D.
-0.6
-0.3
-0.1
growth on ricinoleic acid
YNB Glucose
-0.3
N.D.
-0.3
+0.0
-0.2
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.1
N.D.
+0.3
-0.9
+0.0
ypd buoyancy
40 hrs Low Buoyancy
-0.3
N.D.
-0.3
-0.1
+0.1
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.3
N.D.
-0.1
-0.5
+0.2
ypd buoyancy
40 hrs Median Buoyancy
+0.0
N.D.
-0.6
-0.2
+0.1
auxotrophy
YNB Glucose
-0.4
N.D.
+0.4
-0.3
-0.3
auxotrophy
YNB Glucose 75mM Arginine
-0.2
N.D.
+0.0
-0.0
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.4
N.D.
-0.2
-0.1
+0.2
growth on ypd
YPD
-0.4
N.D.
-0.7
+0.7
+0.0
buoyancy on lipid accumulation
40 hrs Low Buoyancy
+0.3
N.D.
-0.1
-0.7
+0.2
auxotrophy
YNB Glucose 75mM Methionine
-0.2
N.D.
+0.4
-0.1
-0.2
facs on lipid accumulation
Sorted No BODIPY Gate
-0.3
N.D.
+0.1
+0.1
+0.4
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.3
N.D.
+0.4
+0.3
-0.1
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