Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10100 and 10101 are separated by 382 nucleotides10101 and 10102 are separated by 988 nucleotides10102 and 10103 are separated by 963 nucleotides10103 and 10104 are separated by 1303 nucleotides mRNA_1732: 10100 - Hypothetical Protein, at 63,566 to 64,770 10100 mRNA_1733: 10101 - KOG1021 Acetylglucosaminyltransferase EXT1/exostosin 1, at 65,153 to 66,958 10101 mRNA_1734: 10102 - KOG1721 FOG Zn-finger, at 67,947 to 72,087 10102 mRNA_1735: 10103 - K03235 EF3, TEF3 elongation factor 3, at 73,051 to 76,843 10103 mRNA_1736: 10104 - K01593 DDC aromatic-L-amino-acid decarboxylase, at 78,147 to 80,608 10104
Group Condition mRNA_1732 mRNA_1733 mRNA_1734 mRNA_1735 mRNA_1736
10100 10101 10102 10103 10104
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.0 +0.2 -0.0 N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.8 -0.2 +0.0 N.D. -0.1
facs on lipid accumulation Sorted High BODIPY Gate -0.2 -0.1 -0.8 N.D. -0.0
growth on oleic acid YNB Glucose -0.6 -0.3 +0.0 N.D. -0.0
ypd buoyancy 40 hrs No Buoyancy Separation -0.5 +0.0 -0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.9 +0.0 +0.0 N.D. +0.3
ypd buoyancy 40 hrs High Buoyancy -0.3 -0.1 -0.0 N.D. +0.1
auxotrophy Dropout Complete -0.2 -0.5 +0.2 N.D. +0.1
ypd buoyancy 40 hrs Median Buoyancy -0.2 +0.1 -0.0 N.D. -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 -0.1 +0.1 N.D. +0.1
auxotrophy YNB Glucose -0.3 -0.3 +0.2 N.D. +0.1
auxotrophy YNB Glucose 75mM Methionine -0.1 -0.2 +0.3 N.D. -0.1
growth on ricinoleic acid YNB Glucose +0.0 -0.2 +0.2 N.D. -0.1
facs on lipid accumulation Sorted Low BODIPY Gate -0.5 +0.2 +0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.7 +0.2 +0.3 N.D. +0.1
auxotrophy YNB Glucose 75mM Arginine -0.0 -0.1 +0.3 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.4 -0.1 N.D. -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.1 +0.1 N.D. +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.1 -0.1 N.D. +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 +0.2 +0.2 N.D. +0.2
growth on ypd YPD +0.7 +0.0 +0.2 N.D. -0.0
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