Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 10101 and 10102 are separated by 988 nucleotides10102 and 10103 are separated by 963 nucleotides10103 and 10104 are separated by 1303 nucleotides10104 and 10105 are separated by 275 nucleotides mRNA_1733: 10101 - KOG1021 Acetylglucosaminyltransferase EXT1/exostosin 1, at 65,153 to 66,958 10101 mRNA_1734: 10102 - KOG1721 FOG Zn-finger, at 67,947 to 72,087 10102 mRNA_1735: 10103 - K03235 EF3, TEF3 elongation factor 3, at 73,051 to 76,843 10103 mRNA_1736: 10104 - K01593 DDC aromatic-L-amino-acid decarboxylase, at 78,147 to 80,608 10104 mRNA_1737: 10105 - KOG1021 Acetylglucosaminyltransferase EXT1/exostosin 1, at 80,884 to 82,688 10105
Group Condition mRNA_1733 mRNA_1734 mRNA_1735 mRNA_1736 mRNA_1737
10101 10102 10103 10104 10105
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.8 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 -0.0 N.D. -0.2 -0.4
growth on oleic acid YNB Oleic Acid -0.2 +0.0 N.D. -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy +0.1 -0.0 N.D. -0.2 -0.3
growth on oleic acid YNB Glucose -0.3 +0.0 N.D. -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.1 -0.0 N.D. +0.1 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.4 -0.1 N.D. -0.2 -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 +0.0 N.D. +0.3 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 +0.4 N.D. -0.2 -0.3
auxotrophy Dropout Complete -0.5 +0.2 N.D. +0.1 +0.3
auxotrophy YNB Glucose -0.3 +0.2 N.D. +0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.2 +0.3 N.D. -0.1 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 N.D. +0.3 +0.1
growth on ricinoleic acid YNB Glucose -0.2 +0.2 N.D. -0.1 +0.3
auxotrophy YNB Glucose 75mM Arginine -0.1 +0.3 N.D. -0.0 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.0 -0.1 N.D. +0.1 +0.3
growth on ypd YPD +0.0 +0.2 N.D. -0.0 +0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.2 N.D. +0.2 -0.3
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 N.D. +0.1 +0.4
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 +0.3 N.D. +0.1 -0.2
ypd buoyancy 40 hrs Low Buoyancy +0.1 +0.1 N.D. +0.3 +0.0
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